| Entry |
|
|---|
| Name |
benzoate 1,2-dioxygenase;
benzoate hydroxylase;
benzoate hydroxylase;
benzoic hydroxylase;
benzoate dioxygenase;
benzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating,
decarboxylating) [incorrect] |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of two atoms of
oxygen into the other donor
 |
|---|
| Sysname |
benzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating) |
|---|
| Reaction(IUBMB) |
benzoate + NADH + H+ + O2 =
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
[RN:R07188] |
|---|
| Reaction(KEGG) |
R07188 > R05621;
(other) R05622 R08100 R08101 R08108 R08109 R08110
 |
|---|
| Substrate |
benzoate [CPD:C00180];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate [CPD:C06321];
NAD+ [CPD:C00003] |
|---|
| Cofactor |
FAD [CPD:C00016];
Iron [CPD:C00023];
Sulfur [CPD:C00087] |
|---|
| Comment |
A system, containing a reductase which is an iron-sulfur
flavoprotein (FAD), and an iron-sulfur oxygenase. Requires Fe2+. |
|---|
| Pathway |
PATH: ec00362 Benzoate degradation via hydroxylation
PATH: ec00364 Fluorobenzoate degradation
PATH: ec00632 Benzoate degradation via CoA ligation
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K05549 benzoate 1,2-dioxygenase alpha subunit
KO: K05550 benzoate 1,2-dioxygenase beta subunit |
|---|
| Genes |
EFE: EFER_1482(benA) EFER_1483(benB)
KPN: KPN_01871 KPN_01872
KPE: KPK_2484(cbeA) KPK_2485(cbeB)
KPU: KP1_2936(hcaF) KP1_2937(hcaE)
PAE: PA2517(xylY) PA2518(xylX)
PAU: PA14_32080(xylX) PA14_32100(xylY)
PAP: PSPA7_2719(benA) PSPA7_2720(benB)
PAG: PLES_27771(xylX) PLES_27781(xylY)
PPU: PP_3161(benA) PP_3162(benB)
PPF: Pput_2553 Pput_2554
PPG: PputGB1_2687 PputGB1_2688
PPW: PputW619_2709 PputW619_2710
PFL: PFL_3857 PFL_3858
PFO: Pfl01_2321 Pfl01_2967 Pfl01_2968
PFS: PFLU5194 PFLU5195
PEN: PSEEN3142(benB) PSEEN3143(benA)
PSA: PST_1667(benA) PST_1668(benB)
AVN: Avin_08590(benA) Avin_08600(benB)
PCR: Pcryo_1267
ACI: ACIAD1436(benA) ACIAD1437(benB)
ACB: A1S_1214 A1S_1215
ABM: ABSDF1497(benA)
ABY: ABAYE2555(benA) ABAYE2556(benB)
ABC: ACICU_01191 ACICU_01192
ABN: AB57_1349(benB) AB57_1350(benA)
ABB: ABBFA_002364(benA) ABBFA_002365(benB)
RPI: Rpic_1364
RPF: Rpic12D_1428
REU: Reut_B4403
REH: H16_A1962(benB) H16_A1963(benA)
RME: Rmet_4882 Rmet_4883
BMA: BMAA0186(benB) BMAA0187(benA)
BMV: BMASAVP1_1359(benB) BMASAVP1_1360(benA)
BML: BMA10229_1555(benB) BMA10229_1556(benA)
BMN: BMA10247_A0214(benB) BMA10247_A0215(benA)
BPS: BPSS1903(benA) BPSS1904(benB)
BPM: BURPS1710b_A1000(benB)
BPL: BURPS1106A_A2581(benA) BURPS1106A_A2582(benB)
BPD: BURPS668_A2725(benA) BURPS668_A2726(benB)
BTE: BTH_II0472 BTH_II0473
BUR: Bcep18194_C7046
BCM: Bcenmc03_6131
BCJ: BCAS0496(benA)
BAC: BamMC406_6299
BMU: Bmul_3238
BMJ: BMULJ_05287(benA)
BXE: Bxe_B0914(benB) Bxe_B0915(benA)
BPH: Bphy_5351 Bphy_5352
BPY: Bphyt_1591
BGL: bglu_2g21220 bglu_2g21230
BPA: BPP3692
BBR: BB4126
BPT: Bpet1398(benB) Bpet1399(benA)
PNA: Pnap_2112
DAR: Daro_2778 Daro_2779
PLA: Plav_1761 Plav_1762
ARA: Arad_12208 Arad_12209
XAU: Xaut_1134 Xaut_1135
MET: M446_1324 M446_1325
MNO: Mnod_2368 Mnod_2369
NAR: Saro_0538
SWI: Swit_2634 Swit_2635 Swit_4175
MSM: MSMEG_1908
CGL: NCgl2320(cgl2404) NCgl2321(cgl2405)
CGB: cg2637(benA) cg2638(benB)
CGT: cgR_2285 cgR_2286
CEF: CE2305 CE2306
RHA: RHA1_ro02384(benA) RHA1_ro02385(benB)
ROP: ROP_20980(benA) ROP_20990(benB)
GBR: Gbro_4158 Gbro_4159
SEN: SACE_4378(benB) SACE_4379(benA)
 |
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| Structures |
PDB: 3E99 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:214433]
Yamaguchi M, Fujisawa H.
Characterization of NADH-cytochrome c reductase, a component of
benzoate 1,2-dioxygenase system from Pseudomonas arvilla c-1.
J. Biol. Chem. 253 (1978) 8848-53.
Pseudomonas arvilla |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:7372624]
Yamaguchi M, Fujisawa H.
Purification and characterization of an oxygenase component in
benzoate 1,2-dioxygenase system from Pseudomonas arvilla C-1.
J. Biol. Chem. 255 (1980) 5058-63.
Pseudomonas arvilla |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:7130163]
Yamaguchi M, Fujisawa H.
Subunit structure of oxygenase component in benzoate-1,2-dioxygenase
system from Pseudomonas arvilla C-1.
J. Biol. Chem. 257 (1982) 12497-502.
Pseudomonas arvilla |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.12.10
IUBMB Enzyme Nomenclature: 1.14.12.10
ExPASy - ENZYME nomenclature database: 1.14.12.10
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.10
BRENDA, the Enzyme Database: 1.14.12.10
CAS: 9059-18-1 |
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