KEGG   ENZYME: 1.14.12.11Help
Entry
EC 1.14.12.11               Enzyme                                 

Name
toluene dioxygenase;
toluene 2,3-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
toluene + NADH + H+ + O2 = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R03559]
Reaction(KEGG)
R03559;
(other) R09151 R09152
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Substrate
toluene [CPD:C01455];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol [CPD:C04592];
NAD+ [CPD:C00003]
Comment
A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. Some other aromatic compounds, including ethylbenzene, 4-xylene and some halogenated toluenes, are converted into the corresponding cis-dihydrodiols.
History
EC 1.14.12.11 created 1992
Pathway
Toluene degradation
Chloroalkane and chloroalkene degradation
Microbial metabolism in diverse environments
Orthology
K03268  
benzene/toluene/chlorobenzene dioxygenase subunit alpha
K16268  
benzene/toluene/chlorobenzene dioxygenase subunit beta
Genes
PPF: 
PPX: 
T1E_4266(todC1) T1E_4267(todC2)
PMON: 
PMOT: 
BPT: 
Taxonomy
Reference
1  [PMID:16347845]
  Authors
Renganathan V.
  Title
Possible Involvement of Toluene-2,3-Dioxygenase in Defluorination of 3-Fluoro-Substituted Benzenes by Toluene-Degrading Pseudomonas sp. Strain T-12.
  Journal
Appl. Environ. Microbiol. 55 (1989) 330-334.
Reference
2  [PMID:526270]
  Authors
Subramanian V, Liu TN, Yeh WK, Gibson DT.
  Title
Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography.
  Journal
Biochem. Biophys. Res. Commun. 91 (1979) 1131-9.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
120038-36-0

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