KEGG   ENZYME: 1.14.12.11
Entry
EC 1.14.12.11               Enzyme                                 
Name
toluene dioxygenase;
toluene 2,3-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
toluene + NADH + H+ + O2 = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R03559]
Reaction(KEGG)
R03559;
(other) R09151 R09152
Substrate
toluene [CPD:C01455];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol [CPD:C04592];
NAD+ [CPD:C00003]
Comment
A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. Some other aromatic compounds, including ethylbenzene, 4-xylene and some halogenated toluenes, are converted into the corresponding cis-dihydrodiols.
History
EC 1.14.12.11 created 1992
Pathway
ec00623  Toluene degradation
ec00625  Chloroalkane and chloroalkene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03268  benzene/toluene/chlorobenzene dioxygenase subunit alpha
K16268  benzene/toluene/chlorobenzene dioxygenase subunit beta
Genes
PPFPput_2880 Pput_2881
PPXT1E_4266(todC1) T1E_4267(todC2)
PMONX969_10930 X969_10935
PMOTX970_10585 X970_10590
PASILG197_17450 LG197_17455
BPTBpet3738 Bpet3739
ATEMPQV96_18705 PQV96_18710
Reference
1  [PMID:16347845]
  Authors
Renganathan V.
  Title
Possible Involvement of Toluene-2,3-Dioxygenase in Defluorination of 3-Fluoro-Substituted Benzenes by Toluene-Degrading Pseudomonas sp. Strain T-12.
  Journal
Appl Environ Microbiol 55:330-334 (1989)
DOI:10.1128/AEM.55.2.330-334.1989
Reference
2  [PMID:526270]
  Authors
Subramanian V, Liu TN, Yeh WK, Gibson DT.
  Title
Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography.
  Journal
Biochem Biophys Res Commun 91:1131-9 (1979)
DOI:10.1016/0006-291X(79)91998-3
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.11
IUBMB Enzyme Nomenclature: 1.14.12.11
ExPASy - ENZYME nomenclature database: 1.14.12.11
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.11
BRENDA, the Enzyme Database: 1.14.12.11
CAS: 120038-36-0

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