KEGG   ENZYME: 1.14.12.13Help
Entry
EC 1.14.12.13               Enzyme                                 

Name
2-halobenzoate 1,2-dioxygenase;
2-chlorobenzoate 1,2-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
2-halobenzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, dehalogenating, decarboxylating)
Reaction(IUBMB)
a 2-halobenzoate + NADH + H+ + O2 = catechol + a halide anion + NAD+ + CO2 [RN:R01033]
Reaction(KEGG)
Substrate
2-halobenzoate;
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
catechol [CPD:C00090];
halide anion;
NAD+ [CPD:C00003];
CO2 [CPD:C00011]
Comment
A multicomponent enzyme system composed of a dioxygenase component and an electron transfer component. The latter contains FAD. The enzyme, characterized from the bacterium Burkholderia cepacia 2CBS, has a broad substrate specificity. Substrates include 2-fluorobenzoate, 2-chlorobenzoate, 2-bromobenzoate, and 2-iodobenzoate, which are processed in this order of preference.
History
EC 1.14.12.13 created 1992, modified 2012
Pathway
Fluorobenzoate degradation
Microbial metabolism in diverse environments
Orthology
K08686  
2-halobenzoate 1,2-dioxygenase large subunit
K20808  
2-halobenzoate 1,2-dioxygenase small subunit
Reference
1  [PMID:2610934]
  Authors
Fetzner S, Muller R, Lingens F.
  Title
Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS.
  Journal
Biol. Chem. Hoppe. Seyler. 370 (1989) 1173-82.
Reference
2  [PMID:1370284]
  Authors
Fetzner S, Muller R, Lingens F
  Title
Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS.
  Journal
J. Bacteriol. 174 (1992) 279-90.
Reference
3  [PMID:7530709]
  Authors
Haak B, Fetzner S, Lingens F
  Title
Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS.
  Journal
J. Bacteriol. 177 (1995) 667-75.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
125268-83-9

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