KEGG   ENZYME: 1.14.13.103Help
Entry
EC 1.14.13.103              Enzyme                                 

Name
8-dimethylallylnaringenin 2'-hydroxylase;
8-DMAN 2'-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
sophoraflavanone-B,NADPH:oxygen oxidoreductase (2'-hydroxylating)
Reaction(IUBMB)
sophoraflavanone B + NADPH + H+ + O2 = leachianone G + NADP+ + H2O [RN:R08922]
Reaction(KEGG)
Substrate
sophoraflavanone B [CPD:C18023];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
leachianone G [CPD:C18024];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A membrane-bound cytochrome P-450 heme-thiolate monooxygenase that is associated with the endoplasmic reticulum [1,2]. This enzyme is specific for sophoraflavanone B as substrate. NADPH cannot be replaced by NADH, FAD or FMN. Along with EC 2.5.1.70 (naringenin 8-dimethylallyltransferase) and EC 2.5.1.71 (leachianone G 2''-dimethylallyltransferase), this enzyme forms part of the sophoraflavanone-G-biosynthesis pathway.
History
EC 1.14.13.103 created 2007
Reference
1  [PMID:11672730]
  Authors
Yamamoto H, Yatou A, Inoue K.
  Title
8-dimethylallylnaringenin 2'-hydroxylase, the crucial cytochrome P450 mono-oxygenase for lavandulylated flavanone formation in Sophora flavescens cultured cells.
  Journal
Phytochemistry. 58 (2001) 671-6.
Reference
2  [PMID:14551337]
  Authors
Zhao P, Inoue K, Kouno I, Yamamoto H.
  Title
Characterization of leachianone G 2"-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures.
  Journal
Plant. Physiol. 133 (2003) 1306-13.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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