KEGG   ENZYME: 1.14.13.108Help
Entry
EC 1.14.13.108              Enzyme                                 

Name
abieta-7,13-diene hydroxylase;
abietadiene hydroxylase (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
abieta-7,13-diene,NADPH:oxygen oxidoreductase (18-hydroxylating)
Reaction(IUBMB)
abieta-7,13-diene + NADPH + H+ + O2 = abieta-7,13-dien-18-ol + NADP+ + H2O [RN:R06351]
Reaction(KEGG)
Substrate
abieta-7,13-diene;
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
abieta-7,13-dien-18-ol;
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). This enzyme catalyses a step in the pathway of abietic acid biosynthesis. The activity has been demonstrated in cell-free stem extracts of Abies grandis (grand fir) and Pinus contorta (lodgepole pine). The enzyme is localized in the microsomal fraction and requires both oxygen and NADPH. Inhibition by carbon monoxide and several substituted N-heterocyclic inhibitors suggests that the enzyme is a cytochrome P-450-dependent monooxygenase [1]. Activity is induced by wounding of the plant tissue [2].
History
EC 1.14.13.108 created 2009, modified 2012
Pathway
Diterpenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Reference
1  [PMID:8311462]
  Authors
Funk C, Croteau R
  Title
Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis.
  Journal
Arch. Biochem. Biophys. 308 (1994) 258-66.
Reference
2  [PMID:12232380]
  Authors
Funk C, Lewinsohn E, Vogel BS, Steele CL, Croteau R
  Title
Regulation of Oleoresinosis in Grand Fir (Abies grandis) (Coordinate Induction of Monoterpene and Diterpene Cyclases and Two Cytochrome P450-Dependent Diterpenoid Hydroxylases by Stem Wounding).
  Journal
Plant. Physiol. 106 (1994) 999-1005.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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