KEGG   ENZYME: 1.14.13.112Help
Entry
EC 1.14.13.112              Enzyme                                 

Name
3-epi-6-deoxocathasterone 23-monooxygenase;
cytochrome P450 90C1;
CYP90D1;
CYP90C1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-epi-6-deoxocathasterone,NADPH:oxygen oxidoreductase (C-23-hydroxylating)
Reaction(IUBMB)
(1) 3-epi-6-deoxocathasterone + NADPH + H+ + O2 = 6-deoxotyphasterol + NADP+ + H2O [RN:R07792];
(2) (22S,24R)-22-hydroxy-5alpha-ergostan-3-one + NADPH + H+ + O2 = 3-dehydro-6-deoxoteasterone + NADP+ + H2O [RN:R07791]
Reaction(KEGG)
Substrate
3-epi-6-deoxocathasterone [CPD:C16251];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
(22S,24R)-22-hydroxy-5alpha-ergostan-3-one [CPD:C15797]
Product
6-deoxotyphasterol [CPD:C15801];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
3-dehydro-6-deoxoteasterone [CPD:C15800]
Comment
This enzyme is involved in brassinosteroid biosynthesis. C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone and lead directly from (22S,24R)-22-hydroxy-5alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol [1].
History
EC 1.14.13.112 created 2010
Pathway
Brassinosteroid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K12637  
3-epi-6-deoxocathasterone 23-monooxygenase
K12638  
3-epi-6-deoxocathasterone 23-monooxygenase
Genes
ATH: 
AT3G13730(CYP90D1) AT4G36380(ROT3)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
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POP: 
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
DOSA: 
Os05t0200400-01(Os05g0200400)
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
 » show all
Taxonomy
Reference
1  [PMID:17138693]
  Authors
Ohnishi T, Szatmari AM, Watanabe B, Fujita S, Bancos S, Koncz C, Lafos M, Shibata K, Yokota T, Sakata K, Szekeres M, Mizutani M.
  Title
C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis.
  Journal
Plant. Cell. 18 (2006) 3275-88.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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