KEGG   ENZYME: 1.14.13.113Help
Entry
EC 1.14.13.113              Enzyme                                 

Name
FAD-dependent urate hydroxylase;
HpxO enzyme;
FAD-dependent urate oxidase;
urate hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
urate,NADH:oxygen oxidoreductase (5-hydroxyisourate forming)
Reaction(IUBMB)
urate + NADH + H+ + O2 = 5-hydroxyisourate + NAD+ + H2O [RN:R09514]
Reaction(KEGG)
Substrate
urate [CPD:C00366];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
5-hydroxyisourate [CPD:C11821];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
A flavoprotein. The reaction is part of the purine catabolic pathway in the bacterium Klebsiella pneumoniae. The enzyme is different from EC 1.7.3.3, factor-independent urate hydroxylase, found in most plants, which produces hydrogen peroxide. The product of the enzyme is a substrate for EC 3.5.2.17, hydroxyisourate hydrolase.
History
EC 1.14.13.113 created 2010
Pathway
Purine metabolism
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K16839  
FAD-dependent urate hydroxylase
Genes
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PANA_1960(aba2)
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PAJ_1292(aba2)
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 » show all
Taxonomy
Reference
1  [PMID:19260710]
  Authors
O'Leary SE, Hicks KA, Ealick SE, Begley TP
  Title
Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.
  Journal
Biochemistry. 48 (2009) 3033-5.
  Sequence
[kpn:KPN_01663]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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