KEGG   ENZYME: 1.14.13.123Help
Entry
EC 1.14.13.123              Enzyme                                 

Name
germacrene A hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(+)-germacrene-A,NADPH:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
(+)-germacrene A + NADPH + H+ + O2 = germacra-1(10),4,11(13)-trien-12-ol + NADP+ + H2O [RN:R09549]
Reaction(KEGG)
Substrate
(+)-germacrene A [CPD:C16141];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
germacra-1(10),4,11(13)-trien-12-ol [CPD:C19676];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). This is probably part of the biosynthesis of many sesquiterpenoid lactones. In Lactuca sativa EC 1.14.13.123 is a mutifunctional enzyme with EC 1.1.1.314, germacrene A alcohol dehydrogenase [2].
History
EC 1.14.13.123 created 2011
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K15800  
germacrene A oxidase
Reference
1  [PMID:11299372]
  Authors
de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ
  Title
Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid  dehydrogenase(s) involved in sesquiterpene lactone biosynthesis.
  Journal
Plant. Physiol. 125 (2001) 1930-40.
  Sequence
Reference
2  [PMID:20351109]
  Authors
Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK
  Title
Biochemical conservation and evolution of germacrene A oxidase in asteraceae.
  Journal
J. Biol. Chem. 285 (2010) 16588-98.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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