KEGG   ENZYME: 1.14.13.134Help
Entry
EC 1.14.13.134              Enzyme                                 

Name
beta-amyrin 11-oxidase;
CYP88D6
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
beta-amyrin,NADPH:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
beta-amyrin + 2 O2 + 2 NADPH + 2 H+ = 11-oxo-beta-amyrin + 3 H2O + 2 NADP+ (overall reaction) [RN:R09817];
(1a) beta-amyrin + O2 + NADPH + H+ = 11alpha-hydroxy-beta-amyrin + H2O + NADP+ [RN:R09815];
(1b) 11alpha-hydroxy-beta-amyrin + O2 + NADPH + H+ = 11-oxo-beta-amyrin + 2 H2O + NADP+ [RN:R09816]
Reaction(KEGG)
Substrate
beta-amyrin [CPD:C08616];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080];
11alpha-hydroxy-beta-amyrin [CPD:C19942]
Product
11-oxo-beta-amyrin [CPD:C19943];
H2O [CPD:C00001];
NADP+ [CPD:C00006];
11alpha-hydroxy-beta-amyrin [CPD:C19942]
Comment
Requires cytochrome P-450. Part of the glycyrrhizin biosynthesis pathway. The enzyme is also able to oxidize 30-hydroxy-beta-amyrin to 11alpha,30-dihydroxy-beta-amyrin but this is not thought to be part of glycyrrhizin biosynthesis.
History
EC 1.14.13.134 created 2011
Orthology
K17875  
beta-amyrin 11-oxidase
Genes
CAM: 
Taxonomy
Reference
1  [PMID:18779566]
  Authors
Seki H, Ohyama K, Sawai S, Mizutani M, Ohnishi T, Sudo H, Akashi T, Aoki T, Saito K, Muranaka T
  Title
Licorice beta-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 105 (2008) 14204-9.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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