KEGG   ENZYME: 1.14.13.140Help
Entry
EC 1.14.13.140              Enzyme                                 

Name
2-hydroxy-1,4-benzoxazin-3-one monooxygenase;
BX5 (gene name);
CYP71C3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one,NAD(P)H:oxygen oxidoreductase (N-hydroxylating)
Reaction(IUBMB)
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NAD(P)H + H+ + O2 = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + NAD(P)+ + H2O [RN:R07423]
Reaction(KEGG)
Substrate
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one;
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one [CPD:C15770];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses). It is a member of the cytochrome P-450 dependent monooxygenases.
History
EC 1.14.13.140 created 2012
Pathway
Benzoxazinoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K13226  
2-hydroxy-1,4-benzoxazin-3-one monooxygenase
Genes
ATS: 
ZMA: 
Taxonomy
Reference
1  [PMID:16668055]
  Authors
Bailey BA, Larson RL.
  Title
Maize Microsomal Benzoxazinone N-Monooxygenase.
  Journal
Plant. Physiol. 95 (1991) 792-796.
Reference
2  [PMID:10385992]
  Authors
Glawischnig E, Grun S, Frey M, Gierl A.
  Title
Cytochrome P450 monooxygenases of DIBOA biosynthesis: specificity and conservation among grasses.
  Journal
Phytochemistry. 50 (1999) 925-30.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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