KEGG   ENZYME: 1.14.13.143Help
Entry
EC 1.14.13.143              Enzyme                                 

Name
ent-isokaurene C2-hydroxylase;
CYP71Z6
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
ent-isokaurene,NADPH:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
ent-isokaurene + O2 + NADPH + H+ = ent-2alpha-hydroxyisokaurene + H2O + NADP+ [RN:R09861]
Reaction(KEGG)
Substrate
ent-isokaurene [CPD:C20145];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
ent-2alpha-hydroxyisokaurene [CPD:C20146];
H2O [CPD:C00001];
NADP+ [CPD:C00006]
Comment
This is the initial step in the conversion of ent-isokaurene to the antibacterial oryzalides in rice, Oryza sativa.
History
EC 1.14.13.143 created 2012
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K16083  
ent-isokaurene C2-hydroxylase
Genes
OSA: 
4329725(CYP71Z6)
DOSA: 
Os02t0570500-01(Os02g0570500)
Taxonomy
Reference
1  [PMID:21985968]
  Authors
Wu Y, Hillwig ML, Wang Q, Peters RJ
  Title
Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7.
  Journal
FEBS. Lett. 585 (2011) 3446-51.
  Sequence
[osa:4329725]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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