KEGG   ENZYME: 1.14.13.144Help
Entry
EC 1.14.13.144              Enzyme                                 

Name
9beta-pimara-7,15-diene oxidase;
CYP99A3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
9beta-pimara-7,15-diene,NADPH:oxygen 19-oxidoreductase
Reaction(IUBMB)
9beta-pimara-7,15-diene + 3 O2 + 3 NADPH + 3 H+ = 9beta-pimara-7,15-dien-19-oate + 3 NADP+ + 4 H2O (overall reaction) [RN:R09865];
(1a) 9beta-pimara-7,15-diene + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-ol + NADP+ + H2O [RN:R09862];
(1b) 9beta-pimara-7,15-dien-19-ol + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-al + NADP+ + 2 H2O [RN:R09863];
(1c) 9beta-pimara-7,15-dien-19-al + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-oate + NADP+ + H2O [RN:R09864]
Reaction(KEGG)
Substrate
9beta-pimara-7,15-diene [CPD:C18225];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Product
9beta-pimara-7,15-dien-19-oate [CPD:C20149];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Comment
Requires cytochrome P-450. A rice, Oryza sativa, enzyme involved in the phytoalexin momilactone biosynthesis. It also acts similarly on 9beta-stemod-13(17)-ene.
History
EC 1.14.13.144 created 2012
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K16085  
9beta-pimara-7,15-diene oxidase
Genes
OSA: 
4335091(CYP99A3)
DOSA: 
Os04t0178400-01(Os04g0178400)
Taxonomy
Reference
1  [PMID:21175892]
  Authors
Wang Q, Hillwig ML, Peters RJ
  Title
CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice.
  Journal
Plant. J. 65 (2011) 87-95.
  Sequence
[osa:4335091]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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