KEGG   ENZYME: 1.14.13.146Help
Entry
EC 1.14.13.146              Enzyme                                 

Name
taxoid 14beta-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl-acetate,NADPH:oxygen 14-oxidoreductase
Reaction(IUBMB)
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + O2 + NADPH + H+ = 10beta,14beta-dihydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP+ + H2O [RN:R09867]
Reaction(KEGG)
Substrate
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate [CPD:C11898];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
10beta,14beta-dihydroxytaxa-4(20),11-dien-5alpha-yl acetate [CPD:C20151];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Requires cytochrome P450. From the yew Taxus cuspidata. Also acts on taxa-4(20),11-dien-5alpha-yl acetate.
History
EC 1.14.13.146 created 2012
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K20512  
taxoid 14beta-hydroxylase
Reference
1  [PMID:12729625]
  Authors
Jennewein S, Rithner CD, Williams RM, Croteau R
  Title
Taxoid metabolism: Taxoid 14beta-hydroxylase is a cytochrome P450-dependent monooxygenase.
  Journal
Arch. Biochem. Biophys. 413 (2003) 262-70.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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