KEGG   ENZYME: 1.14.13.152Help
Entry
EC 1.14.13.152              Enzyme                                 

Name
geraniol 8-hydroxylase;
CYP76B6;
G10H;
CrG10H;
SmG10H
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
geraniol,NADPH:oxygen oxidoreductase (8-hydroxylating)
Reaction(IUBMB)
geraniol + NADPH + H+ + O2 = (6E)-8-hydroxygeraniol + NADP+ + H2O [RN:R08785]
Reaction(KEGG)
Substrate
geraniol [CPD:C01500];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(6E)-8-hydroxygeraniol [CPD:C17621];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Requires cytochrome P-450. Also hydroxylates nerol and citronellol, cf. EC 1.14.13.151 linalool 8-monooxygenase. The recommended numbering of geraniol gives 8-hydroxygeraniol as the product rather than 10-hydroxygeraniol as used by references 1-3. See prenol nomenclature {iupac/misc/prenol#p1::Pr-1}. The cloned enzyme also catalysed, but less efficiently, the 3'-hydroxylation of naringenin (cf. EC 1.14.13.21, flavonoid 3'-monooxygenase) [3].
History
EC 1.14.13.152 created 2012
Pathway
Monoterpenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K15099  
geraniol 8-hydroxylase
Reference
1  [PMID:11718718]
  Authors
Collu G, Unver N, Peltenburg-Looman AM, van der Heijden R, Verpoorte R, Memelink J.
  Title
Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis.
  Journal
FEBS. Lett. 508 (2001) 215-20.
  Sequence
Reference
2  [PMID:20699579]
  Authors
Wang J, Liu Y, Cai Y, Zhang F, Xia G, Xiang F
  Title
Cloning and functional analysis of geraniol 10-hydroxylase, a cytochrome P450 from Swertia mussotii Franch.
  Journal
Biosci. Biotechnol. Biochem. 74 (2010) 1583-90.
  Sequence
Reference
3  [PMID:21504162]
  Authors
Sung PH, Huang FC, Do YY, Huang PL
  Title
Functional expression of geraniol 10-hydroxylase reveals its dual function in the biosynthesis of terpenoid and phenylpropanoid.
  Journal
J. Agric. Food. Chem. 59 (2011) 4637-43.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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