KEGG   ENZYME: 1.14.13.158Help
Entry
EC 1.14.13.158              Enzyme                                 

Name
amorpha-4,11-diene 12-monooxygenase;
CYP71AV1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
amorpha-4,11-diene,NADPH:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+ = artemisinate + 3 NADP+ + 4 H2O (overall reaction) [RN:R10026];
(1a) amorpha-4,11-diene + O2 + NADPH + H+ = artemisinic alcohol + NADP+ + H2O [RN:R10023];
(1b) artemisinic alcohol + O2 + NADPH + H+ = artemisinic aldehyde + NADP+ + 2 H2O [RN:R10024];
(1c) artemisinic aldehyde + O2 + NADPH + H+ = artemisinate + NADP+ + H2O [RN:R10025]
Reaction(KEGG)
Substrate
amorpha-4,11-diene [CPD:C16028];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080];
artemisinic alcohol [CPD:C20307];
artemisinic aldehyde [CPD:C20308]
Product
artemisinate [CPD:C20309];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
artemisinic alcohol [CPD:C20307];
artemisinic aldehyde [CPD:C20308]
Comment
A heme-thiolate protein (P-450). Cloned from the plant Artemisia annua (sweet wormwood). Part of the biosynthetic pathway of artemisinin.
History
EC 1.14.13.158 created 2012
Orthology
K21716  
amorpha-4,11-diene 12-monooxygenase
Reference
1  [PMID:16458889]
  Authors
Teoh KH, Polichuk DR, Reed DW, Nowak G, Covello PS
  Title
Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin.
  Journal
FEBS. Lett. 580 (2006) 1411-6.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system