Entry |
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Name |
amorpha-4,11-diene 12-monooxygenase;
CYP71AV1
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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Sysname |
amorpha-4,11-diene,NADPH:oxygen oxidoreductase (12-hydroxylating)
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Reaction(IUBMB) |
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+ = artemisinate + 3 NADP+ + 4 H2O (overall reaction) [RN: R10026];
(1a) amorpha-4,11-diene + O2 + NADPH + H+ = artemisinic alcohol + NADP+ + H2O [RN: R10023];
(1b) artemisinic alcohol + O2 + NADPH + H+ = artemisinic aldehyde + NADP+ + 2 H2O [RN: R10024];
(1c) artemisinic aldehyde + O2 + NADPH + H+ = artemisinate + NADP+ + H2O [RN: R10025]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
A heme-thiolate protein (P-450). Cloned from the plant Artemisia annua (sweet wormwood). Part of the biosynthetic pathway of artemisinin.
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History |
EC 1.14.13.158 created 2012
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Orthology |
| amorpha-4,11-diene 12-monooxygenase |
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Reference |
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Authors |
Teoh KH, Polichuk DR, Reed DW, Nowak G, Covello PS |
Title |
Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin. |
Journal |
FEBS. Lett. 580 (2006) 1411-6. |
Sequence |
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Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |