KEGG   ENZYME: 1.14.13.163Help
Entry
EC 1.14.13.163              Enzyme                                 

Name
6-hydroxy-3-succinoylpyridine 3-monooxygenase;
6-hydroxy-3-succinoylpyridine hydroxylase;
hspA (gene name);
hspB (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate,NADH:oxygen oxidoreductase (3-hydroxylating, succinate semialdehyde releasing)
Reaction(IUBMB)
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 = 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O [RN:R09472]
Reaction(KEGG)
Substrate
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate [CPD:C19631];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxypyridine [CPD:C01059];
succinate semialdehyde [CPD:C00232];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme catalyses a reaction in the nicotine degradation pathway of Pseudomonas species. One of the enzymes from the soil bacterium Pseudomonas putida S16 contains an FAD cofactor [2].
History
EC 1.14.13.163 created 2012
Pathway
Nicotinate and nicotinamide metabolism
Orthology
K15359  
6-hydroxy-3-succinoylpyridine 3-monooxygenase
K18276  
6-hydroxy-3-succinoylpyridine 3-monooxygenase
Genes
PPT: 
PPUH: 
PMON: 
PMOT: 
Taxonomy
Reference
1  [PMID:18203859]
  Authors
Tang H, Wang S, Ma L, Meng X, Deng Z, Zhang D, Ma C, Xu P
  Title
A novel gene, encoding 6-hydroxy-3-succinoylpyridine hydroxylase, involved in nicotine degradation by Pseudomonas putida strain S16.
  Journal
Appl. Environ. Microbiol. 74 (2008) 1567-74.
  Organism
Pseudomonas putida
  Sequence
[ppt:PPS_0380]
Reference
2  [PMID:21949128]
  Authors
Tang H, Yao Y, Zhang D, Meng X, Wang L, Yu H, Ma L, Xu P
  Title
A novel NADH-dependent and FAD-containing hydroxylase is crucial for nicotine degradation by Pseudomonas putida.
  Journal
J. Biol. Chem. 286 (2011) 39179-87.
  Organism
Pseudomonas putida
  Sequence
[ppt:PPS_4061]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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