KEGG   ENZYME: 1.14.13.172Help
Entry
EC 1.14.13.172              Enzyme                                 

Name
salicylate 5-hydroxylase;
nagG (gene name);
nagH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
salicylate,NADH:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
salicylate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R07709]
Reaction(KEGG)
R07709;
(other) R07710
Show
Substrate
salicylate [CPD:C00805];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
This enzyme, which was characterized from the bacterium Ralstonia sp. U2, comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.7, ferredoxin---NAD(P)+ reductase), an iron-sulfur oxygenase, and ferredoxin.
History
EC 1.14.13.172 created 2013
Pathway
Naphthalene degradation
Microbial metabolism in diverse environments
Orthology
K18242  
salicylate 5-hydroxylase large subunit
K18243  
salicylate 5-hydroxylase small subunit
Genes
EBT: 
PLU: 
PTT: 
ALT: 
TTU: 
MMW: 
MME: 
MPC: 
RSO: 
RSc1089(nagH) RSc1090(nagG)
RSL: 
RSN: 
RSM: 
RSE: 
F504_1060(ohbA) F504_1061(ohbB)
RPJ: 
RMN: 
REU: 
REH: 
H16_A1632(nagH) H16_A1633(nagG)
CNC: 
RME: 
Rmet_3712(nagH)
CBW: 
BMK: 
BPYR: 
BGL: 
BGU: 
BUG: 
BUB: 
BUQ: 
BPX: 
BFN: 
PPK: 
PPNO: 
PPNM: 
PRB: 
PPUL: 
PSPU: 
PAPI: 
PVE: 
POX: 
PFG: 
PNR: 
BPT: 
AXY: 
AXO: 
AXN: 
AXS: 
ADT: 
POL: 
PNA: 
AAV: 
AJS: 
AAA: 
ACK: 
VAP: 
VPE: 
VPD: 
RTA: 
LIM: 
LIH: 
MASW: 
LCH: 
AZO: 
azo2515(nagH) azo2516(nagG)
SWI: 
AZL: 
ALI: 
TMO: 
 » show all
Taxonomy
Reference
1  [PMID:9573207]
  Authors
Fuenmayor SL, Wild M, Boyes AL, Williams PA
  Title
A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.
  Journal
J. Bacteriol. 180 (1998) 2522-30.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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