KEGG   ENZYME: 1.14.13.173Help
Entry
EC 1.14.13.173              Enzyme                                 

Name
11-oxo-beta-amyrin 30-oxidase;
CYP72A;
CYP72A154
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
11-oxo-beta-amyrin,NADPH:oxygen oxidoreductase (30-hydroxylating)
Reaction(IUBMB)
11-oxo-beta-amyrin + 3 O2 + 3 NADPH + 3 H+ = glycyrrhetinate + 3 NADP+ + 4 H2O (overall reaction) [RN:R10316];
(1a) 11-oxo-beta-amyrin + O2 + NADPH + H+ = 30-hydroxy-11-oxo-beta-amyrin + NADP+ + H2O [RN:R10313];
(1b) 30-hydroxy-11-oxo-beta-amyrin + O2 + NADPH + H+ = glycyrrhetaldehyde + NADP+ + 2 H2O [RN:R10314];
(1c) glycyrrhetaldehyde + O2 + NADPH + H+ = glycyrrhetinate + NADP+ + H2O [RN:R10315]
Reaction(KEGG)
Substrate
11-oxo-beta-amyrin [CPD:C19943];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080];
30-hydroxy-11-oxo-beta-amyrin [CPD:C20510];
glycyrrhetaldehyde [CPD:C20511]
Product
glycyrrhetinate [CPD:C02283];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
30-hydroxy-11-oxo-beta-amyrin [CPD:C20510];
glycyrrhetaldehyde [CPD:C20511]
Comment
A heme-thiolate protein (cytochrome P-450). The enzyme is involved in the biosynthesis of the triterpenoid saponin glycyrrhizin in the plant Glycyrrhiza uralensis (licorice). The enzyme from the plant Medicago truncatula can also hydroxylate beta-amyrin.
History
EC 1.14.13.173 created 2013
Orthology
K17873  
11-oxo-beta-amyrin 30-oxidase
Genes
MTR: 
CAM: 
Taxonomy
Reference
1  [PMID:22128119]
  Authors
Seki H, Sawai S, Ohyama K, Mizutani M, Ohnishi T, Sudo H, Fukushima EO, Akashi T, Aoki T, Saito K, Muranaka T
  Title
Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin.
  Journal
Plant. Cell. 23 (2011) 4112-23.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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