KEGG   ENZYME: 1.14.13.180Help
Entry
EC 1.14.13.180              Enzyme                                 

Name
aklavinone 12-hydroxylase;
DnrF;
RdmE;
aklavinone 11-hydroxylase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
aklavinone,NADPH:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
aklavinone + NADPH + H+ + O2 = epsilon-rhodomycinone + NADP+ + H2O [RN:R06683]
Reaction(KEGG)
Substrate
aklavinone [CPD:C12424];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
epsilon-rhodomycinone [CPD:C12425];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzymes from the Gram-positive bacteria Streptomyces peucetius and Streptomyces purpurascens participate in the biosynthesis of daunorubicin, doxorubicin and rhodomycins. The enzyme from Streptomyces purpurascens is an FAD monooxygenase.
History
EC 1.14.13.180 created 2013
Pathway
Biosynthesis of type II polyketide products
Biosynthesis of antibiotics
Orthology
K15950  
aklavinone 12-hydroxylase
Reference
1  [PMID:7773378]
  Authors
Filippini S, Solinas MM, Breme U, Schluter MB, Gabellini D, Biamonti G, Colombo AL, Garofano L
  Title
Streptomyces peucetius daunorubicin biosynthesis gene, dnrF: sequence and heterologous expression.
  Journal
Microbiology. 141 ( Pt 4) (1995) 1007-16.
  Sequence
Reference
2  [PMID:10082933]
  Authors
Niemi J, Wang Y, Airas K, Ylihonko K, Hakala J, Mantsala P
  Title
Characterization of aklavinone-11-hydroxylase from Streptomyces purpurascens.
  Journal
Biochim. Biophys. Acta. 1430 (1999) 57-64.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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