KEGG   ENZYME: 1.14.13.183Help
Entry
EC 1.14.13.183              Enzyme                                 

Name
dammarenediol 12-hydroxylase;
protopanaxadiol synthase;
CYP716A47;
dammarenediol-II,NADPH:O2 oxidoreductase (12beta-hydroxylating)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
dammarenediol-II,NADPH:oxygen oxidoreductase (12beta-hydroxylating)
Reaction(IUBMB)
dammarenediol-II + NADPH + H+ + O2 = protopanaxadiol + NADP+ + H2O [RN:R10540]
Reaction(KEGG)
Substrate
dammarenediol II [CPD:C19829];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
protopanaxadiol [CPD:C20715];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Involved in the biosynthetic pathway of ginsenosides. Isolated from ginseng (Panax ginseng).
History
EC 1.14.13.183 created 2013
Orthology
K20545  
dammarenediol 12-hydroxylase
Reference
1  [PMID:22039120]
  Authors
Han JY, Kim HJ, Kwon YS, Choi YE
  Title
The Cyt P450 enzyme CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-II during ginsenoside biosynthesis in Panax ginseng.
  Journal
Plant. Cell. Physiol. 52 (2011) 2062-73.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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