KEGG   ENZYME: 1.14.13.184Help
Entry
EC 1.14.13.184              Enzyme                                 

Name
protopanaxadiol 6-hydroxylase;
protopanaxatriol synthase;
P6H;
CYP716A53v2;
protopanaxadiol,NADPH:O2 oxidoreductase (6alpha-hydroxylating)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
protopanaxadiol,NADPH:oxygen oxidoreductase (6alpha-hydroxylating)
Reaction(IUBMB)
protopanaxadiol + NADPH + H+ + O2 = protopanaxatriol + NADP+ + H2O [RN:R10591]
Reaction(KEGG)
Substrate
protopanaxadiol [CPD:C20715];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
protopanaxatriol [CPD:C20716];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Involved in the biosynthetic pathway of ginsenosides. Isolated from the rhizomes of ginseng (Panax ginseng).
History
EC 1.14.13.184 created 2013
Orthology
K20546  
protopanaxadiol 6-hydroxylase
Reference
1  [PMID:18351680]
  Authors
Yue CJ, Zhou X, Zhong JJ
  Title
Protopanaxadiol 6-hydroxylase and its role in regulating the ginsenoside heterogeneity in Panax notoginseng cells.
  Journal
Biotechnol. Bioeng. 100 (2008) 933-40.
Reference
2  [PMID:22875608]
  Authors
Han JY, Hwang HS, Choi SW, Kim HJ, Choi YE
  Title
Cytochrome P450 CYP716A53v2 catalyzes the formation of protopanaxatriol from protopanaxadiol during ginsenoside biosynthesis in Panax ginseng.
  Journal
Plant. Cell. Physiol. 53 (2012) 1535-45.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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