KEGG   ENZYME: 1.14.13.186Help
Entry
EC 1.14.13.186              Enzyme                                 

Name
20-oxo-5-O-mycaminosyltylactone 23-monooxygenase;
tylH1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
20-oxo-5-O-beta-mycaminosyltylactone,NADPH:oxygen oxidoreductase (23-hydroxylating)
Reaction(IUBMB)
20-oxo-5-O-beta-mycaminosyltylactone + NADPH + H+ + O2 = 5-O-beta-mycaminosyltylonolide + NADP+ + H2O [RN:R10656]
Reaction(KEGG)
Substrate
20-oxo-5-O-beta-mycaminosyltylactone [CPD:C20760];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
5-O-beta-mycaminosyltylonolide;
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A cytochrome P-450 heme-thiolate enzyme. Involved in the biosynthetic pathway of the macrolide antibiotic tylosin, which is produced by several species of Streptomyces bacteria.
History
EC 1.14.13.186 created 2014
Pathway
Biosynthesis of 12-, 14- and 16-membered macrolides
Type I polyketide structures
Biosynthesis of antibiotics
Orthology
K15991  
20-oxo-5-O-mycaminosyltylactone 23-monooxygenase
Reference
1  [PMID:7283418]
  Authors
Baltz RH, Seno ET
  Title
Properties of Streptomyces fradiae mutants blocked in biosynthesis of the macrolide antibiotic tylosin.
  Journal
Antimicrob. Agents. Chemother. 20 (1981) 214-25.
Reference
2  [PMID:15489173]
  Authors
Reeves CD, Ward SL, Revill WP, Suzuki H, Marcus M, Petrakovsky OV, Marquez S, Fu H, Dong SD, Katz L
  Title
Production of hybrid 16-membered macrolides by expressing combinations of polyketide synthase genes in engineered Streptomyces fradiae hosts.
  Journal
Chem. Biol. 11 (2004) 1465-72.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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