KEGG   ENZYME: 1.14.13.190Help
Entry
EC 1.14.13.190              Enzyme                                 

Name
ferruginol synthase;
miltiradiene oxidase (incorrect);
CYP76AH1;
miltiradiene,NADPH:oxygen oxidoreductase (ferruginol forming) (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
miltiradiene,NADPH:oxygen oxidoreductase (ferruginol-forming)
Reaction(IUBMB)
abieta-8,11,13-triene + NADPH + H+ + O2 = ferruginol + NADP+ + H2O [RN:R10727]
Reaction(KEGG)
Substrate
abieta-8,11,13-triene;
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
ferruginol [CPD:C09092];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme is found in some members of the Lamiaceae (mint family). The enzyme from Rosmarinus officinalis (rosemary) is involved in biosynthesis of carnosic acid, while the enzyme from the Chinese medicinal herb Salvia miltiorrhiza is involved in the biosynthesis of the tanshinones, abietane-type norditerpenoid naphthoquinones that are the main lipophilic bioactive components found in the plant.
History
EC 1.14.13.190 created 2014, modified 2015
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K20513  
ferruginol synthase
Genes
SIND: 
Taxonomy
Reference
1  [PMID:23812755]
  Authors
Guo J, Zhou YJ, Hillwig ML, Shen Y, Yang L, Wang Y, Zhang X, Liu W, Peters RJ, Chen X, Zhao ZK, Huang L
  Title
CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 12108-13.
  Sequence
Reference
2  [PMID:24108414]
  Authors
Zi J, Peters RJ
  Title
Characterization of CYP76AH4 clarifies phenolic diterpenoid biosynthesis in the Lamiaceae.
  Journal
Org. Biomol. Chem. 11 (2013) 7650-2.
Reference
3  [PMID:26020634]
  Authors
Bozic D, Papaefthimiou D, Bruckner K, de Vos RC, Tsoleridis CA, Katsarou D, Papanikolaou A, Pateraki I, Chatzopoulou FM, Dimitriadou E, Kostas S, Manzano D, Scheler U, Ferrer A, Tissier A, Makris AM, Kampranis SC, Kanellis AK
  Title
Towards Elucidating Carnosic Acid Biosynthesis in Lamiaceae: Functional Characterization of the Three First Steps of the Pathway in Salvia fruticosa and  Rosmarinus officinalis.
  Journal
PLoS. One. 10 (2015) e0124106.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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