KEGG   ENZYME: 1.14.13.197Help
Entry
EC 1.14.13.197              Enzyme                                 

Name
dihydromonacolin L hydroxylase;
LovA (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
dihydromonacolin L acid,NADPH:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
dihydromonacolin L acid + O2 + NADPH + H+ = monacolin L acid + NADP+ + 2 H2O (overall reaction) [RN:R10793];
(1a) dihydromonacolin L acid + O2 + NADPH + H+ = 3alpha-hydroxy-3,5-dihydromonacolin L acid + NADP+ + H2O [RN:R10791];
(1b) 3alpha-hydroxy-3,5-dihydromonacolin L acid = monacolin L acid + H2O (spontaneous) [RN:R10792]
Reaction(KEGG)
Substrate
dihydromonacolin L acid [CPD:C20851];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080];
3alpha-hydroxy-3,5-dihydromonacolin L acid [CPD:C20852]
Product
monacolin L acid [CPD:C20853];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
3alpha-hydroxy-3,5-dihydromonacolin L acid [CPD:C20852]
Comment
A heme-thiolate protein (cytochrome P-450). The dehydration of 3alpha-hydroxy-3,5-dihydromonacolin L acid is believed to be spontaneous [1,2]. The enzyme from fungi also catalyses the reaction of EC 1.14.13.198, monacolin L hydroxylase [3].
History
EC 1.14.13.197 created 2014
Reference
1  [PMID:2921224]
  Authors
Treiber LR, Reamer RA, Rooney CS, Ramjit HG
  Title
Origin of monacolin L from Aspergillus terreus cultures.
  Journal
J. Antibiot. (Tokyo). 42 (1989) 30-6.
Reference
2  [PMID:2276977]
  Authors
Nakamura T, Komagata D, Murakawa S, Sakai K, Endo A
  Title
Isolation and biosynthesis of 3 alpha-hydroxy-3,5-dihydromonacolin L.
  Journal
J. Antibiot. (Tokyo). 43 (1990) 1597-600.
Reference
3  [PMID:21495633]
  Authors
Barriuso J, Nguyen DT, Li JW, Roberts JN, MacNevin G, Chaytor JL, Marcus SL, Vederas JC, Ro DK
  Title
Double oxidation of the cyclic nonaketide dihydromonacolin L to monacolin J by a  single cytochrome P450 monooxygenase, LovA.
  Journal
J. Am. Chem. Soc. 133 (2011) 8078-81.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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