| Entry |
|
|---|
| Name |
2,4-dichlorophenol 6-monooxygenase;
2,4-dichlorophenol hydroxylase;
2,4-dichlorophenol monooxygenase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
|---|
| Sysname |
2,4-dichlorophenol,NADPH:oxygen oxidoreductase (6-hydroxylating) |
|---|
| Reaction(IUBMB) |
2,4-dichlorophenol + NADPH + H+ + O2 = 3,5-dichlorocatechol + NADP+
+ H2O [RN:R03997] |
|---|
| Reaction(KEGG) |
R03997;
(other) R05441
 |
|---|
| Substrate |
2,4-dichlorophenol [CPD:C02625];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
3,5-dichlorocatechol [CPD:C02933];
NADP+ [CPD:C00006];
H2O [CPD:C00001] |
|---|
| Cofactor |
FAD [CPD:C00016] |
|---|
| Comment |
A flavoprotein (FAD). Also acts, more slowly, on 4-chlorophenol and
4-chloro-2-methylphenol; NADH can act instead of NADPH, but more
slowly. |
|---|
| Pathway |
PATH: ec00627 1,4-Dichlorobenzene degradation
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K10676 2,4-dichlorophenol 6-monooxygenase |
|---|
| Genes |
OLU: OSTLU_87314
TET: TTHERM_00637700
ACB: A1S_1079
REU: Reut_D6462 Reut_D6470
BUR: Bcep18194_A3910
DAR: Daro_0899
RLE: pRL120094(tfdB)
BRA: BRADO2059 BRADO2271(tfdB)
BBT: BBta_2389
RHA: RHA1_ro00538 RHA1_ro03910 RHA1_ro10313
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:7075592]
Beadle CA, Smith AR.
The purification and properties of 2,4-dichlorophenol hydroxylase
from a strain of Acinetobacter species.
Eur. J. Biochem. 123 (1982) 323-32.
Acinetobacter sp. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.20
IUBMB Enzyme Nomenclature: 1.14.13.20
ExPASy - ENZYME nomenclature database: 1.14.13.20
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.20
BRENDA, the Enzyme Database: 1.14.13.20
CAS: 82047-82-3 |
|---|
