KEGG   ENZYME: 1.14.13.23Help
Entry
EC 1.14.13.23               Enzyme                                 

Name
3-hydroxybenzoate 4-monooxygenase;
3-hydroxybenzoate 4-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-hydroxybenzoate,NADPH:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
3-hydroxybenzoate + NADPH + H+ + O2 = 3,4-dihydroxybenzoate + NADP+ + H2O [RN:R01628]
Reaction(KEGG)
Substrate
3-hydroxybenzoate [CPD:C00587];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3,4-dihydroxybenzoate [CPD:C00230];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions.
History
EC 1.14.13.23 created 1972 as EC 1.14.99.13, transferred 1984 to EC 1.14.13.23
Pathway
Benzoate degradation
Polycyclic aromatic hydrocarbon degradation
Microbial metabolism in diverse environments
Orthology
K19065  
3-hydroxybenzoate 4-monooxygenase
Genes
CTT: 
CTES: 
Taxonomy
Reference
1  [PMID:4148586]
  Authors
Michalover JL, Ribbons DW, Hughes H.
  Title
3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni.
  Journal
Biochem. Biophys. Res. Commun. 55 (1973) 888-96.
Reference
2  [PMID:4390252]
  Authors
Premkumar R, Rao PV, Sreeleela NS, Vaidyanathan CS.
  Title
m-Hydroxybenzoic acid 4-hydroxylase from Aspergillus niger.
  Journal
Can. J. Biochem. 47 (1969) 825-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
37256-76-1

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