KEGG   ENZYME: 1.14.13.31Help
Entry
EC 1.14.13.31               Enzyme                                 

Name
2-nitrophenol 2-monooxygenase;
2-nitrophenol oxygenase;
nitrophenol oxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
2-nitrophenol,NADPH:oxygen 2-oxidoreductase (2-hydroxylating, nitrite-forming)
Reaction(IUBMB)
2-nitrophenol + 2 NADPH + 2 H+ + O2 = catechol + nitrite + 2 NADP+ + H2O [RN:R00828]
Reaction(KEGG)
Substrate
2-nitrophenol [CPD:C01988];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
catechol [CPD:C00090];
nitrite [CPD:C00088];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Involved in the metabolism of nitro-aromatic compounds by a strain of Pseudomonas putida.
History
EC 1.14.13.31 created 1989
Reference
1  [PMID:3752997]
  Authors
Zeyer J, Kocher HP, Timmis KN.
  Title
Influence of para-substituents on the oxidative metabolism of o-nitrophenols by Pseudomonas putida B2.
  Journal
Appl. Environ. Microbiol. 52 (1986) 334-9.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
104520-84-5

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