KEGG   ENZYME: 1.14.13.38Help
Entry
EC 1.14.13.38               Enzyme                                 

Name
anhydrotetracycline 6-monooxygenase;
ATC oxygenase;
anhydrotetracycline oxygenase;
oxyS (gene name);
anhydrotetracycline monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
anhydrotetracycline,NADPH:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
anhydrotetracycline + NADPH + H+ + O2 = 12-dehydrotetracycline + NADP+ + H2O [RN:R04060]
Reaction(KEGG)
R04060;
(other) R09193
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Substrate
anhydrotetracycline [CPD:C02811];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
12-dehydrotetracycline [CPD:C03206];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. It can also catalyse EC 1.14.13.234, 12-dehydrotetracycline 5-monooxygenase.
History
EC 1.14.13.38 created 1990, modified 2016
Pathway
Tetracycline biosynthesis
Biosynthesis of type II polyketide products
Biosynthesis of antibiotics
Orthology
K14256  
anhydrotetracycline 6-monooxygenase / 5a,11a-dehydrotetracycline 5-monooxygenase
Reference
1
  Authors
Behal, V., Hostalek, Z. and Vanek, Z.
  Title
Anhydrotetracycline oxygenase activity and biosynthesis of tetracyclines in streptomyces aureofaciens.
  Journal
Biotechnol. Lett. 1 (1979) 177-182.
Reference
2  [PMID:2914874]
  Authors
Binnie C, Warren M, Butler MJ
  Title
Cloning and heterologous expression in Streptomyces lividans of Streptomyces rimosus genes involved in oxytetracycline biosynthesis.
  Journal
J. Bacteriol. 171 (1989) 887-95.
Reference
3  [PMID:3138982]
  Authors
Vancurova I, Volc J, Flieger M, Neuzil J, Novotna J, Vlach J, Behal V
  Title
Isolation of pure anhydrotetracycline oxygenase from Streptomyces aureofaciens.
  Journal
Biochem. J. 253 (1988) 263-7.
Reference
4  [PMID:23621493]
  Authors
Wang P, Bashiri G, Gao X, Sawaya MR, Tang Y
  Title
Uncovering the enzymes that catalyze the final steps in oxytetracycline biosynthesis.
  Journal
J. Am. Chem. Soc. 135 (2013) 7138-41.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
70766-62-0

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