KEGG   ENZYME: 1.14.13.4Help
Entry
EC 1.14.13.4                Enzyme                                 

Name
melilotate 3-monooxygenase;
2-hydroxyphenylpropionate hydroxylase;
melilotate hydroxylase;
2-hydroxyphenylpropionic hydroxylase;
melilotic hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-(2-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
3-(2-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O [RN:R03369]
Reaction(KEGG)
Substrate
3-(2-hydroxyphenyl)propanoate [CPD:C01198];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3-(2,3-dihydroxyphenyl)propanoate [CPD:C04044];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD).
History
EC 1.14.13.4 created 1972
Pathway
Phenylalanine metabolism
Microbial metabolism in diverse environments
Reference
1  [PMID:6017743]
  Authors
Levy CC.
  Title
Melilotate hydroxylase. Purification of the enzyme and the nature of the prosthetic group.
  Journal
J. Biol. Chem. 242 (1967) 747-53.
Reference
2  [PMID:4285850]
  Authors
Levy CC, Frost P.
  Title
The metabolism of coumarin by a microorganism. V. Melilotate hydroxylase.
  Journal
J. Biol. Chem. 241 (1966) 997-1003.
Reference
3  [PMID:4348920]
  Authors
Strickland S, Massey V.
  Title
The purification and properties of the flavoprotein melilotate hydroxylase.
  Journal
J. Biol. Chem. 248 (1973) 2944-52.
Reference
4  [PMID:4348921]
  Authors
Strickland S, Massey V.
  Title
The mechanism of action of the flavoprotein melilotate hydroxylase.
  Journal
J. Biol. Chem. 248 (1973) 2953-62.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37256-72-7

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