KEGG   ENZYME: 1.14.13.63Help
Entry
EC 1.14.13.63               Enzyme                                 

Name
3-hydroxyphenylacetate 6-hydroxylase;
3-hydroxyphenylacetate 6-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
3-hydroxyphenylacetate + NAD(P)H + H+ + O2 = 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O [RN:R02515 R02517]
Reaction(KEGG)
Substrate
3-hydroxyphenylacetate [CPD:C05593];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxyphenylacetate [CPD:C00544];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
3-hydroxyphenylacetate 6-hydroxylase from Flavobacterium sp. is highly specific for 3-hydroxyphenylacetate and uses NADH and NADPH as electron donors with similar efficiency.
History
EC 1.14.13.63 created 1999
Pathway
ec00643  Styrene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K10438  3-hydroxyphenylacetate 6-hydroxylase
Genes
SLB: AWJ20_2761 AWJ20_5196(ERG5)
MGR: MGG_03352
FGR: FGSG_06447
FPU: FPSE_10099
FVR: FVEG_04417
FOX: FOXG_07489 FOXG_10783
NHE: NECHADRAFT_53600 NECHADRAFT_87111
MAW: MAC_02148
MAJ: MAA_00010 MAA_04437 MAA_06936
CMT: CCM_02612 CCM_07933
ANI: AN1397.2
ANG: ANI_1_12074(An08g00180)
CIM: CIMG_13120(CIMG07204)
PBL: PAAG_11575(PAAG_02928) PAAG_11576(PAAG_02928)
ABE: ARB_02605
TVE: TRV_05771
PTE: PTT_06974
 » show all
Taxonomy
Reference
1  [PMID:1935954]
  Authors
Van Berkel WJ, Van Den Tweel WJ.
  Title
Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species.
  Journal
Eur. J. Biochem. 201 (1991) 585-92.
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.63
IUBMB Enzyme Nomenclature: 1.14.13.63
ExPASy - ENZYME nomenclature database: 1.14.13.63
BRENDA, the Enzyme Database: 1.14.13.63
CAS: 114705-01-0

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