KEGG   ENZYME: 1.14.13.63Help
Entry
EC 1.14.13.63               Enzyme                                 
Name
3-hydroxyphenylacetate 6-hydroxylase;
3-hydroxyphenylacetate 6-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
3-hydroxyphenylacetate + NAD(P)H + H+ + O2 = 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O [RN:R02515 R02517]
Reaction(KEGG)
Substrate
3-hydroxyphenylacetate [CPD:C05593];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxyphenylacetate [CPD:C00544];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
3-hydroxyphenylacetate 6-hydroxylase from Flavobacterium sp. is highly specific for 3-hydroxyphenylacetate and uses NADH and NADPH as electron donors with similar efficiency.
Pathway
Styrene degradation
Microbial metabolism in diverse environments
Orthology
K10438  
3-hydroxyphenylacetate 6-hydroxylase
Genes
MGR: 
FGR: 
ANI: 
NFI: 
AFM: 
AOR: 
AOR_1_2894174(AO090005001647)
ANG: 
ANI_1_12074(An08g00180)
ACT: 
PCS: 
CIM: 
CPW: 
CPC735_035300
URE: 
UREG_05702
PNO: 
SNOG_13913
 » show all
Taxonomy
Reference
1  [PMID:1935954]
  Authors
Van Berkel WJ, Van Den Tweel WJ.
  Title
Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species.
  Journal
Eur. J. Biochem. 201 (1991) 585-92.
  Organism
Flavobacterium sp.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
114705-01-0
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