KEGG   ENZYME: 1.14.13.76Help
Entry
EC 1.14.13.76               Enzyme                                 

Name
taxane 10beta-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
taxa-4(20),11-dien-5alpha-yl acetate,NADPH:oxygen oxidoreductase (10beta-hydroxylating)
Reaction(IUBMB)
taxa-4(20),11-dien-5alpha-yl acetate + NADPH + H+ + O2 = 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP+ + H2O [RN:R06309]
Reaction(KEGG)
Substrate
taxa-4(20),11-dien-5alpha-yl acetate;
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate [CPD:C11898];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
This microsomal cytochrome-P-450-dependent enzyme is involved in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel).
History
EC 1.14.13.76 created 2002
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K12924  
taxane 10-beta-hydroxylase
Reference
1  [PMID:11396929]
  Authors
Wheeler AL, Long RM, Ketchum RE, Rithner CD, Williams RM, Croteau R.
  Title
Taxol biosynthesis: differential transformations of taxadien-5 alpha-ol and its acetate ester by cytochrome P450 hydroxylases from Taxus suspension cells.
  Journal
Arch. Biochem. Biophys. 390 (2001) 265-78.
Reference
2  [PMID:11707604]
  Authors
Jennewein S, Rithner CD, Williams RM, Croteau RB.
  Title
Taxol biosynthesis: taxane 13 alpha-hydroxylase is a cytochrome P450-dependent monooxygenase.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 13595-600.
Reference
3  [PMID:11171980]
  Authors
Schoendorf A, Rithner CD, Williams RM, Croteau RB.
  Title
Molecular cloning of a cytochrome P450 taxane 10 beta-hydroxylase cDNA from Taxus and functional expression in yeast.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 1501-6.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
337514-75-7

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