KEGG   ENZYME: 1.14.13.77Help
Entry
EC 1.14.13.77               Enzyme                                 

Name
taxane 13alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
taxa-4(20),11-dien-5alpha-ol,NADPH:oxygen oxidoreductase (13alpha-hydroxylating)
Reaction(IUBMB)
taxa-4(20),11-dien-5alpha-ol + NADPH + H+ + O2 = taxa-4(20),11-dien-5alpha,13alpha-diol + NADP+ + H2O [RN:R06308]
Reaction(KEGG)
Substrate
taxa-4(20),11-dien-5alpha-ol [CPD:C11895];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
taxa-4(20),11-dien-5alpha,13alpha-diol;
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
This cytochrome-P-450-dependent enzyme is involved in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel).
History
EC 1.14.13.77 created 2002
Pathway
Diterpenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K12922  
taxane 13-alpha-hydroxylase
Reference
1  [PMID:11396929]
  Authors
Wheeler AL, Long RM, Ketchum RE, Rithner CD, Williams RM, Croteau R.
  Title
Taxol biosynthesis: differential transformations of taxadien-5 alpha-ol and its acetate ester by cytochrome P450 hydroxylases from Taxus suspension cells.
  Journal
Arch. Biochem. Biophys. 390 (2001) 265-78.
Reference
2  [PMID:11707604]
  Authors
Jennewein S, Rithner CD, Williams RM, Croteau RB.
  Title
Taxol biosynthesis: taxane 13 alpha-hydroxylase is a cytochrome P450-dependent monooxygenase.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 13595-600.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
399030-58-1

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