KEGG   ENZYME: 1.14.13.78Help
Entry
EC 1.14.13.78               Enzyme                                 

Name
ent-kaurene oxidase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
ent-kaur-16-ene + 3 NADPH + 3 H+ + 3 O2 = ent-kaur-16-en-19-oate + 3 NADP+ + 4 H2O (overall reaction) [RN:R10066];
(1a) ent-kaur-16-ene + NADPH + H+ + O2 = ent-kaur-16-en-19-ol + NADP+ + H2O [RN:R06291];
(1b) ent-kaur-16-en-19-ol + NADPH + H+ + O2 = ent-kaur-16-en-19-al + NADP+ + 2 H2O [RN:R06292];
(1c) ent-kaur-16-en-19-al + NADPH + O2 = ent-kaur-16-en-19-oate + NADP+ + H2O [RN:R06293]
Reaction(KEGG)
Substrate
ent-kaur-16-ene [CPD:C06090];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Product
ent-kaur-16-en-19-oate [CPD:C11874];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Comment
Requires cytochrome P-450. Catalyses three sucessive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
History
EC 1.14.13.78 created 2002
Pathway
Diterpenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K04122  
ent-kaurene oxidase
Genes
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
100802970(CYP701A16) 100814223
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj0g3v0151459.1(Lj0g3v0151459.1) Lj0g3v0180559.1(Lj0g3v0180559.1) Lj0g3v0218449.1(Lj0g3v0218449.1) Lj3g3v0002210.2(Lj3g3v0002210.2)
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
DOSA: 
Os06t0568600-01(Os06g0568600) Os06t0569500-01(Os06g0569500) Os06t0569900-01(Os06g0569900) Os06t0570100-01(Os06g0570100) Os06t0570566-00(Os06g0570566) Os06t0570600-00(Os06g0570600)
OBR: 
BDI: 
ATS: 
SBI: 
SORBI_10g022520(SORBIDRAFT_10g022520)
ZMA: 
100274015(GRMZM2G059308) 103638615(GRMZM2G161472)
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
 » show all
Taxonomy
Reference
1  [PMID:2223832]
  Authors
Ashman PJ, Mackenzie A, Bramley PM.
  Title
Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi.
  Journal
Biochim. Biophys. Acta. 1036 (1990) 151-7.
Reference
2  [PMID:1397591]
  Authors
Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM.
  Title
Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima.
  Journal
Biochem. Soc. Trans. 20 (1992) 218S.
Reference
3  [PMID:9952446]
  Authors
Helliwell CA, Poole A, Peacock WJ, Dennis ES.
  Title
Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.
  Journal
Plant. Physiol. 119 (1999) 507-10.
  Sequence
[ath:AT5G25900]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
149565-67-3

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