KEGG   ENZYME: 1.14.13.79Help
Entry
EC 1.14.13.79               Enzyme                                 

Name
ent-kaurenoic acid oxidase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2 = gibberellin A12 + 3 NADP+ + 4 H2O (overall reaction) [RN:R10067];
(1a) ent-kaur-16-en-19-oate + NADPH + H+ + O2 = ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O [RN:R06294];
(1b) ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H+ + O2 = gibberellin A12 aldehyde + NADP+ + 2 H2O [RN:R06295];
(1c) gibberellin A12 aldehyde + NADPH + H+ + O2 = gibberellin A12 + NADP+ + H2O [RN:R06297]
Reaction(KEGG)
Substrate
ent-kaur-16-en-19-oate [CPD:C11874];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
ent-7alpha-hydroxykaur-16-en-19-oate [CPD:C11875];
gibberellin A12 aldehyde [CPD:C06093]
Product
gibberellin A12 [CPD:C11857];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
ent-7alpha-hydroxykaur-16-en-19-oate [CPD:C11875];
gibberellin A12 aldehyde [CPD:C06093]
Comment
Requires cytochrome P-450. Catalyses three sucessive oxidations of ent-kaurenoic acid. The second step includes a ring-B contraction giving the gibbane skeleton. In pumpkin (Cucurbita maxima) ent-6alpha,7alpha-dihydroxykaur-16-en-19-oate is also formed.
History
EC 1.14.13.79 created 2002
Pathway
Diterpenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K04123  
ent-kaurenoic acid hydroxylase
Genes
ATH: 
AT1G05160(CYP88A3) AT2G32440(KAO2)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj2g3v1985180.1(Lj2g3v1985180.1) Lj3g3v0730100.1(Lj3g3v0730100.1) Lj3g3v1983610.1(Lj3g3v1983610.1) Lj3g3v1983620.1(Lj3g3v1983620.1) Lj3g3v1983620.2(Lj3g3v1983620.2) Lj3g3v1993700.1(Lj3g3v1993700.1) Lj3g3v1993710.1(Lj3g3v1993710.1) Lj4g3v1152920.1(Lj4g3v1152920.1) Lj6g3v1618120.1(Lj6g3v1618120.1) Lj6g3v1618120.2(Lj6g3v1618120.2) Lj6g3v1618120.3(Lj6g3v1618120.3)
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0013s15760g(POPTRDRAFT_571955) POPTR_0014s17720g(POPTRDRAFT_688380)
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
4339885(KAO)
DOSA: 
Os06t0110000-01(Os06g0110000)
OBR: 
BDI: 
ATS: 
SBI: 
SORBI_10g000920(SORBIDRAFT_10g000920)
ZMA: 
103639316(GRMZM2G089803) 542580(d3)
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
 » show all
Taxonomy
Reference
1  [PMID:11172076]
  Authors
Helliwell CA, Chandler PM, Poole A, Dennis ES, Peacock WJ.
  Title
The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 2065-70.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
337507-95-6

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