KEGG   ENZYME: 1.14.13.89Help
Entry
EC 1.14.13.89               Enzyme                                 

Name
isoflavone 2'-hydroxylase;
isoflavone 2'-monooxygenase;
CYP81E1;
CYP Ge-3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
isoflavone,NADPH:oxygen oxidoreductase (2'-hydroxylating)
Reaction(IUBMB)
an isoflavone + NADPH + H+ + O2 = a 2'-hydroxyisoflavone + NADP+ + H2O [RN:R07371]
Reaction(KEGG)
Substrate
isoflavone [CPD:C00799];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2'-hydroxyisoflavone [CPD:C02921];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Acts on daidzein, formononetin and genistein. EC 1.14.13.53, 4'-methoxyisoflavone 2'-hydroxylase, has the same reaction but is more specific as it requires a 4'-methoxyisoflavone.
History
EC 1.14.13.89 created 2005
Pathway
Isoflavonoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K13260  
isoflavone 2'-hydroxylase
Genes
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
Taxonomy
Reference
1  [PMID:9790908]
  Authors
Akashi T, Aoki T, Ayabe S.
  Title
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.
  Journal
Biochem. Biophys. Res. Commun. 251 (1998) 67-70.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
110183-49-8

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