KEGG   ENZYME: 1.14.13.94Help
Entry
EC 1.14.13.94               Enzyme                                 

Name
lithocholate 6beta-hydroxylase;
lithocholate 6beta-monooxygenase;
CYP3A10;
6beta-hydroxylase;
cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
lithocholate,NADPH:oxygen oxidoreductase (6beta-hydroxylating)
Reaction(IUBMB)
lithocholate + NADPH + H+ + O2 = 6beta-hydroxylithocholate + NADP+ + H2O [RN:R07203]
Reaction(KEGG)
Substrate
lithocholate [CPD:C03990];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
6beta-hydroxylithocholate [CPD:C15515];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Expression of the gene for this enzyme is 50-fold higher in male compared to female hamsters [1].
History
EC 1.14.13.94 created 2005
Reference
1  [PMID:1840595]
  Authors
Teixeira J, Gil G.
  Title
Cloning, expression, and regulation of lithocholic acid 6 beta-hydroxylase.
  Journal
J. Biol. Chem. 266 (1991) 21030-6.
Reference
2  [PMID:8484723]
  Authors
Chang TK, Teixeira J, Gil G, Waxman DJ.
  Title
The lithocholic acid 6 beta-hydroxylase cytochrome P-450, CYP 3A10, is an active catalyst of steroid-hormone 6 beta-hydroxylation.
  Journal
Biochem. J. 291 ( Pt 2) (1993) 429-33.
Reference
3  [PMID:9547277]
  Authors
Subramanian A, Wang J, Gil G.
  Title
STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase.
  Journal
Nucleic. Acids. Res. 26 (1998) 2173-8.
Reference
4  [PMID:12543708]
  Authors
Russell DW.
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu. Rev. Biochem. 72 (2003) 137-74.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9075-83-6

DBGET integrated database retrieval system