KEGG   ENZYME: 1.14.14.12Help
Entry
EC 1.14.14.12               Enzyme                                 

Name
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase;
HsaA
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione,FMNH2:oxygen oxidoreductase
Reaction(IUBMB)
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O [RN:R09819]
Reaction(KEGG)
Substrate
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C19944];
FMNH2 [CPD:C01847];
O2 [CPD:C00007]
Product
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C04793];
FMN [CPD:C00061];
H2O [CPD:C00001]
Comment
This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. It can use either FADH or FMNH2 as flavin cofactor. The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.
History
EC 1.14.14.12 created 2011
Pathway
Steroid degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K16047  
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Genes
XDO: 
PRE: 
PPUT: 
PPUN: 
PFE: 
PPUU: 
PKC: 
PFZ: 
PPSY: 
AVN: 
AVL: 
AVD: 
ACX: 
SPL: 
SHL: 
PHA: 
PTN: 
ZAL: 
GPB: 
REU: 
REH: 
H16_B0638(h16_B0638)
CNC: 
CTI: 
CBW: 
CGD: 
CR3_0799(hsaA)
BUR: 
BCJ: 
BCEN: 
BCEO: 
BCT: 
BCED: 
BPYR: 
BCON: 
BUG: 
BGE: 
BGO: 
BUQ: 
BPX: 
BFN: 
PVE: 
POX: 
CTT: 
CTES: 
AZI: 
DAR: 
THU: 
SUR: 
AVI: 
BJA: 
BJU: 
BJP: 
BRS: 
BRC: 
BRAD: 
NWI: 
NHA: 
XAU: 
RHZ: 
CSE: 
NAR: 
NPP: 
SPHK: 
SWI: 
SPHM: 
STAX: 
SPHI: 
SSAN: 
SSY: 
SBD: 
BSM: 
PNP: 
MTU: 
Rv3570c(hsaA)
MTV: 
MTC: 
MRA: 
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTI: 
MTE: 
MTUR: 
MTL: 
MTO: 
MTD: 
MTN: 
MTJ: 
MTUB: 
MTUC: 
MTUE: 
MTX: 
MTUH: 
MTUL: 
MTUT: 
MTUU: 
MTQ: 
MBO: 
MBB: 
MBT: 
MBM: 
MBK: 
MBX: 
MBZ: 
MAF: 
MCE: 
MCQ: 
MCV: 
MCX: 
MCZ: 
MMIC: 
MPA: 
MAO: 
MAVI: 
MAVU: 
MAV: 
MAVD: 
MAVR: 
MAVA: 
MIT: 
MIR: 
MIA: 
MIE: 
MID: 
MYO: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MUL: 
MVA: 
MGI: 
MSP: 
MAB: 
MABB: 
MMV: 
MAY: 
MABO: 
MABL: 
MAZ: 
MAK: 
MYS: 
MYC: 
MMC: 
MKM: 
MJL: 
MJD: 
MMI: 
MRH: 
MMM: 
MCB: 
MLI: 
MKN: 
MKS: 
MKI: 
MNE: 
MYV: 
MYE: 
MGO: 
MFT: 
MHAD: 
MPHL: 
MVQ: 
ASD: 
NFA: 
NCY: 
NBR: 
NNO: 
RHA: 
RER: 
REY: 
REB: 
ROP: 
ROA: 
REQ: 
RPY: 
RAV: 
GBR: 
GPO: 
GOR: 
KTR9_0820(hsaA)
GOQ: 
TPR: 
SCB: 
SALU: 
SALL: 
SVT: 
STRM: 
NCA: 
PSIM: 
TCU: 
SRO: 
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
AJA: 
AMQ: 
ALU: 
KAL: 
KPHY: 
STP: 
SAQ: 
AMS: 
TBI: 
 » show all
Taxonomy
Reference
1  [PMID:20448045]
  Authors
Dresen C, Lin LY, D'Angelo I, Tocheva EI, Strynadka N, Eltis LD
  Title
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.
  Journal
J. Biol. Chem. 285 (2010) 22264-75.
  Sequence
[mtu:Rv3570c]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 

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