KEGG   ENZYME: 1.14.14.7Help
Entry
EC 1.14.14.7                Enzyme                                 
Name
tryptophan 7-halogenase;
PrnA;
RebH
Class
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
L-tryptophan:FADH2 oxidoreductase (7-halogenating)
Reaction(IUBMB)
tryptophan + FADH2 + Cl- + O2 + H+ = 7-chloro-L-tryptophan + FAD + 2 H2O [RN:R09570]
Reaction(KEGG)
Substrate
tryptophan [CPD:C00078];
FADH2 [CPD:C01352];
Cl- [CPD:C00698];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
7-chloro-L-tryptophan [CPD:C19687];
FAD [CPD:C00016];
H2O [CPD:C00001]
Comment
In Lechevalieria aerocolonigenes the enzyme catalyses the initial step in the biosynthesis of rebeccamycin [2].
Orthology
K14266  
FADH2 O2-dependent halogenase I
Genes
RCU: 
SRR: 
SRS: 
SRA: 
SMAF: 
XCC: 
XCC3966(prnA) XCC4133(prnA)
XCB: 
XCA: 
XCV: 
XCP: 
XAC: 
XAC4275(prnA)
XAX: 
XACM_3918 XACM_4146
XAO: 
XAC29_20405
XOO: 
XOO0248(prnA)
XOM: 
XOO_0227
XOP: 
PXO_03613
XOR: 
XOC_4642
XAL: 
XALc_0046
XCI: 
XCAW_00251(prnA) XCAW_00399(prnA)
PSU: 
Psesu_0608
PSD: 
DSC_13765
RHD: 
R2APBS1_3097
PFL: 
PFL_3604(prnA)
CJA: 
CJA_1070 CJA_1159 CJA_2881 CJA_3150
SON: 
SO_3513
SDN: 
Sden_2030 Sden_2707
SFR: 
Sfri_1309 Sfri_1786
SAZ: 
Sama_0939
SBL: 
Sbal_1121
SBM: 
Shew185_1176 Shew185_2037
SBN: 
Sbal195_1172 Sbal195_1209 Sbal195_2084
SBP: 
Sbal223_2301 Sbal223_3181 Sbal223_3217
SBT: 
Sbal678_1200 Sbal678_1239 Sbal678_2086
SBS: 
Sbal117_1180 Sbal117_1222
SBB: 
Sbal175_3152 Sbal175_3194
SLO: 
Shew_1113 Shew_2293
SSE: 
Ssed_1209
SPL: 
Spea_1098
SHE: 
Shewmr4_2941
SHM: 
Shewmr7_3023
SHN: 
Shewana3_3120
SHL: 
Shal_1146
SWD: 
Swoo_1140 Swoo_1306 Swoo_2208 Swoo_3937
SWP: 
swp_1626
SVO: 
SVI_1019 SVI_1518
CPS: 
CPS_0992 CPS_3700 CPS_3734
PHA: 
PSHAa1353
PAT: 
Patl_3277 Patl_4289
PSM: 
PSM_A1379 PSM_A1883
SDE: 
Sde_1096 Sde_1401
AMC: 
MADE_1009520
AMAC: 
MASE_09130 MASE_19100 MASE_19660
AMB: 
AMBAS45_09685
AMG: 
AMEC673_09460 AMEC673_19415
AMK: 
AMBLS11_08935 AMBLS11_18405 AMBLS11_18850
AMAA: 
amad1_10325 amad1_19710
ALT: 
ambt_07620 ambt_22070
GAG: 
Glaag_1076 Glaag_2305 Glaag_3730 Glaag_4254
GNI: 
GNIT_3223
GPS: 
C427_0190 C427_2235 C427_3390
TTU: 
TERTU_0510 TERTU_1076 TERTU_2326
FBL: 
Fbal_1097 Fbal_2925
KKO: 
Kkor_1103 Kkor_1104
SAGA: 
M5M_04370 M5M_05405 M5M_05410 M5M_10725 M5M_18335
BPM: 
BURPS1710b_A1115(prnA)
BPL: 
BURPS1106A_A2733
BPD: 
BURPS668_A2885
BPQ: 
BPC006_II2696
BUR: 
Bcep18194_C6706
BCM: 
Bcenmc03_6983
BAM: 
Bamb_4726
BAC: 
BamMC406_5264
RFR: 
Rfer_1114
LCH: 
Lcho_3228
BBA: 
Bd1390(prnA)
BBAT: 
Bdt_1370(prnA)
MSD: 
MYSTI_03287 MYSTI_03293
RHI: 
NGR_a00820
SFH: 
SFHH103_04336
SFD: 
USDA257_p02540
MEX: 
Mext_2404
MEA: 
Mex_1p2407
MPO: 
Mpop_2363
CCR: 
CC_0787 CC_0972 CC_1529 CC_1755 CC_2805 CC_2807 CC_2808
CCS: 
CCNA_00828 CCNA_01023 CCNA_01054 CCNA_01598 CCNA_01831 CCNA_02371 CCNA_02896 CCNA_02898 CCNA_02899
CAK: 
Caul_1290 Caul_1839 Caul_1840 Caul_1842 Caul_2064 Caul_4092
CSE: 
Cseg_2051 Cseg_2053 Cseg_2054 Cseg_3271 Cseg_3732
PZU: 
PHZ_c2922
BSB: 
Bresu_2902 Bresu_2932
AEX: 
Astex_0704 Astex_1893 Astex_1988 Astex_2586 Astex_2721 Astex_3306 Astex_3307 Astex_3309 Astex_3382 Astex_3384
PGA: 
PGA1_c26580
PGL: 
PGA2_c24610
MMR: 
Mmar10_2721 Mmar10_2723 Mmar10_2724 Mmar10_2733 Mmar10_2734
HBA: 
Hbal_2452 Hbal_2461 Hbal_2462
NAR: 
Saro_1882
NPP: 
PP1Y_AT20776
SAL: 
Sala_0182 Sala_0740
SPHM: 
G432_14600
SJP: 
SJA_C2-02280
ELI: 
ELI_03165
MMI: 
MMAR_3632
SHY: 
SHJG_8627
SHO: 
SHJGH_8387
SVE: 
SVEN_6229
SEN: 
SACE_4919(prnA)
SESP: 
BN6_32440(prnA)
CYP: 
PCC8801_1365
CYH: 
Cyan8802_1395
NOP: 
Nos7524_0715
CSG: 
Cylst_4395
 » show all
Taxonomy
Reference
1  [PMID:15272170]
  Authors
Dong C, Kotzsch A, Dorward M, van Pee KH, Naismith JH
  Title
Crystallization and X-ray diffraction of a halogenating enzyme, tryptophan 7-halogenase, from Pseudomonas fluorescens.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 60 (2004) 1438-40.
  Organism
Pseudomonas fluorescens
Reference
2  [PMID:15743914]
  Authors
Yeh E, Garneau S, Walsh CT
  Title
Robust in vitro activity of RebF and RebH, a two-component reductase/halogenase,  generating 7-chlorotryptophan during rebeccamycin biosynthesis.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 3960-5.
Reference
3
  Authors
Bitto, E., Huang, Y., Bingman, C.A., Singh, S., Thorson, J.S. and Phillips Jr., G.N.
  Title
The structure of flavin-dependent tryptophan 7-halogenase RebH.
  Journal
Proteins Struct. Funct. Genet. 70 (2008) 289-293.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system