KEGG   ENZYME: 1.14.14.7Help
Entry
EC 1.14.14.7                Enzyme                                 

Name
tryptophan 7-halogenase;
PrnA;
RebH
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
L-tryptophan:FADH2 oxidoreductase (7-halogenating)
Reaction(IUBMB)
tryptophan + FADH2 + Cl- + O2 + H+ = 7-chloro-L-tryptophan + FAD + 2 H2O [RN:R09570]
Reaction(KEGG)
Substrate
tryptophan [CPD:C00078];
FADH2 [CPD:C01352];
Cl- [CPD:C00698];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
7-chloro-L-tryptophan [CPD:C19687];
FAD [CPD:C00016];
H2O [CPD:C00001]
Comment
In Lechevalieria aerocolonigenes the enzyme catalyses the initial step in the biosynthesis of rebeccamycin [2].
History
EC 1.14.14.7 created 2009
Orthology
K14266  
FADH2 O2-dependent halogenase I
Genes
RCU: 
SRR: 
SRL: 
SRY: 
SRS: 
SRA: 
SMAF: 
XCC: 
XCC3966(prnA) XCC4133(prnA)
XCB: 
XCA: 
XCP: 
XCV: 
XAC: 
XAC4275(prnA)
XCI: 
XAX: 
XAO: 
XOO: 
XOO0248(prnA) XOO0391(prnA)
XOM: 
XOP: 
XOR: 
XAL: 
XFU: 
PSU: 
PSD: 
RHD: 
DJI: 
PFL: 
PFL_3604(prnA)
PPRC: 
PCH: 
CJA: 
SON: 
SDN: 
SFR: 
SAZ: 
SBL: 
SBM: 
SBN: 
SBP: 
SBT: 
SBS: 
SBB: 
SLO: 
SSE: 
SPL: 
SHE: 
SHM: 
SHN: 
SHL: 
SWD: 
SWP: 
SVO: 
CPS: 
PHA: 
PAT: 
PSM: 
SDE: 
AMC: 
AMAC: 
AMB: 
AMG: 
AMH: 
AMK: 
AMAA: 
AMAL: 
AMAE: 
AMAO: 
AMAD: 
AMAI: 
AMAG: 
ALT: 
AAL: 
GAG: 
GNI: 
GPS: 
TTU: 
FBL: 
KKO: 
SAGA: 
BPM: 
BPL: 
BPD: 
BPSE: 
BDL_5422(prnA)
BPSM: 
BBQ_4121(prnA)
BPSU: 
BBN_5475(prnA)
BPSD: 
BBX_4589(prnA)
BPQ: 
BPK: 
BBK_4751(prnA)
BTZ: 
BTL_5470(prnA)
BUR: 
BCM: 
BAM: 
BAC: 
RFR: 
LCH: 
BBA: 
Bd1390(prnA)
BBAT: 
Bdt_1370(prnA)
BBAC: 
MSD: 
RHI: 
SFH: 
SFD: 
MEX: 
MEA: 
MPO: 
CCR: 
CCS: 
CAK: 
CSE: 
PZU: 
BSB: 
AEX: 
PGA: 
PGL: 
PGD: 
MMR: 
HBA: 
NAR: 
NPP: 
SAL: 
SPHM: 
SJP: 
ELI: 
MMI: 
SHY: 
SHO: 
SVE: 
SCI: 
SEN: 
SACE_4919(prnA)
AOI: 
AORI_5336(prnA)
SESP: 
KAL: 
CYP: 
CYH: 
NOP: 
CSG: 
SALI: 
 » show all
Taxonomy
Reference
1  [PMID:15272170]
  Authors
Dong C, Kotzsch A, Dorward M, van Pee KH, Naismith JH
  Title
Crystallization and X-ray diffraction of a halogenating enzyme, tryptophan 7-halogenase, from Pseudomonas fluorescens.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 60 (2004) 1438-40.
  Sequence
[up:P95480]
Reference
2  [PMID:15743914]
  Authors
Yeh E, Garneau S, Walsh CT
  Title
Robust in vitro activity of RebF and RebH, a two-component reductase/halogenase,  generating 7-chlorotryptophan during rebeccamycin biosynthesis.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 3960-5.
  Sequence
[up:Q8KHZ8]
Reference
3
  Authors
Bitto, E., Huang, Y., Bingman, C.A., Singh, S., Thorson, J.S. and Phillips Jr., G.N.
  Title
The structure of flavin-dependent tryptophan 7-halogenase RebH.
  Journal
Proteins Struct. Funct. Genet. 70 (2008) 289-293.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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