KEGG   ENZYME: 1.14.15.13Help
Entry
EC 1.14.15.13               Enzyme                                 

Name
pulcherriminic acid synthase;
cyclo-L-leucyl-L-leucyl dipeptide oxidase;
CYP134A1;
CypX (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
cyclo(L-leucyl-L-leucyl),reduced-ferredoxin:oxygen oxidoreductase (N-hydroxylating,aromatizing)
Reaction(IUBMB)
cyclo(L-leucyl-L-leucyl) + 6 reduced ferredoxin + 3 O2 = pulcherriminic acid + 6 oxidized ferredoxin + 4 H2O [RN:R10322]
Reaction(KEGG)
Substrate
cyclo(L-leucyl-L-leucyl) [CPD:C20514];
reduced ferredoxin [CPD:C00138];
O2 [CPD:C00007]
Product
pulcherriminic acid [CPD:C20515];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001]
Comment
A heme-thiolate (P-450) enzyme from the bacterium Bacillus subtilis. The order of events during the overall reaction is unknown. Pulcherrimic acid spontaneously forms an iron chelate with Fe(3+) to form the red pigment pulcherrimin [2].
History
EC 1.14.15.13 created 2013
Orthology
K17474  
pulcherriminic acid synthase
Genes
PLU: 
MME: 
BSU: 
BSU35060(cypX)
BSH: 
BSUT: 
BSUL: 
BSUS: 
BSS: 
BST: 
BSQ: 
BSP: 
BLI: 
BL00818(cypX)
BLD: 
BLi03567(cypX)
BLH: 
BACL: 
BALM: 
SHA: 
SSD: 
SDT: 
SPSE_0788(cypX)
PDX: 
 » show all
Taxonomy
Reference
1  [PMID:5837792]
  Authors
MacDonald JC
  Title
Biosynthesis of pulcherriminic acid.
  Journal
Biochem. J. 96 (1965) 533-8.
Reference
2  [PMID:20690619]
  Authors
Cryle MJ, Bell SG, Schlichting I
  Title
Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase.
  Journal
Biochemistry. 49 (2010) 7282-96.
  Sequence
[bsu:BSU35060]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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