KEGG   ENZYME: 1.14.15.9Help
Entry
EC 1.14.15.9                Enzyme                                 

Name
spheroidene monooxygenase;
CrtA;
acyclic carotenoid 2-ketolase;
spirilloxantin monooxygenase;
2-oxo-spirilloxanthin monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
spheroidene,reduced-ferredoxin:oxygen oxidoreductase (spheroiden-2-one-forming)
Reaction(IUBMB)
(1) spheroidene + reduced ferredoxin + O2 = spheroiden-2-one + oxidized ferredoxin + H2O [RN:R07537];
(2) spirilloxantin + reduced ferredoxin + O2 = 2-oxospirilloxanthin + oxidized ferredoxin + H2O [RN:R07525];
(3) 2'-oxospirilloxanthin + reduced ferredoxin + O2 = 2,2'-dioxospirilloxanthin + oxidized ferredoxin + H2O [RN:R07526]
Reaction(KEGG)
Substrate
spheroidene [CPD:C15900];
reduced ferredoxin [CPD:C00138];
O2 [CPD:C00007];
spirilloxantin;
2-oxospirilloxanthin [CPD:C15884]
Product
spheroiden-2-one [CPD:C15903];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001];
2-oxospirilloxanthin [CPD:C15884];
2,2'-dioxospirilloxanthin [CPD:C15885]
Comment
The enzyme is involved in spheroidenone biosynthesis and in 2,2'-dioxospirilloxanthin biosynthesis. The enzyme from Rhodobacter sphaeroides contains heme at its active site [1].
History
EC 1.14.15.9 created 2012
Pathway
Carotenoid biosynthesis
Metabolic pathways
Orthology
K09847  
spheroidene monooxygenase
Genes
RSP: 
RSP_0272(crtA)
RSH: 
RSQ: 
RSK: 
RCP: 
JAN: 
Jann_0144(crtA)
RDE: 
RD1_0121(crtA)
RLI: 
DSH: 
Dshi_3501(crtA)
Taxonomy
Reference
1  [PMID:20851979]
  Authors
Lee PC, Holtzapple E, Schmidt-Dannert C
  Title
Novel activity of Rhodobacter sphaeroides spheroidene monooxygenase CrtA expressed in Escherichia coli.
  Journal
Appl. Environ. Microbiol. 76 (2010) 7328-31.
  Sequence
Reference
2  [PMID:19136077]
  Authors
Gerjets T, Steiger S, Sandmann G
  Title
Catalytic properties of the expressed acyclic carotenoid 2-ketolases from Rhodobacter capsulatus and Rubrivivax gelatinosus.
  Journal
Biochim. Biophys. Acta. 1791 (2009) 125-31.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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