KEGG   ENZYME: 1.14.19.6Help
Entry
EC 1.14.19.6                Enzyme                                 

Name
acyl-CoA (9+3)-desaturase;
oleoyl-CoA 12-desaturase;
Delta12 fatty acid desaturase;
Delta12(omega6)-desaturase;
oleoyl-CoA Delta12 desaturase;
Delta12 desaturase;
Delta12-desaturase;
Delta12-fatty-acid desaturase;
acyl-CoA,hydrogen donor:oxygen Delta12-oxidoreductase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
(1) oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11043];
(2) palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11044]
Reaction(KEGG)
Substrate
oleoyl-CoA [CPD:C00510];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
palmitoleoyl-CoA [CPD:C21072]
Product
linoleoyl-CoA [CPD:C02050];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA [CPD:C21000]
Comment
This microsomal enzyme introduces a cis double bond at position 12 of fatty-acyl-CoAs that contain a cis double bond at position 9. When acting on 19:1Delta10 fatty acyl-CoA the enzyme from the pathogenic protozoan Trypanosoma brucei introduces the new double bond at position 13, indicating that the new double bond is introduced three carbons from the existing cis double bond, towards the methyl-end of the fatty acid. Requires cytochrome b5 as the electron donor [4].
History
EC 1.14.19.6 created 2008, modified 2015
Pathway
Biosynthesis of unsaturated fatty acids
Orthology
K10256  
omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Genes
NVE: 
ATH: 
AT3G12120(FAD2)
ALY: 
CRB: 
EUS: 
BRP: 
103870276(FAD2-1)
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
8267086(FAD2) 8267537(FAH12)
JCU: 
POP: 
POPTR_0001s04990g(POPTRDRAFT_639715) POPTR_0001s05000g POPTR_0001s05020g(POPTRDRAFT_797040) POPTR_0006s20640g(POPTRDRAFT_561258) POPTR_0016s04550g(POPTRDRAFT_667376)
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
DOSA: 
Os02t0716500-01(Os02g0716500) Os07t0416900-01(Os07g0416900) Os07t0417200-01(Os07g0417200)
OBR: 
BDI: 
SBI: 
SORBI_02g003840(SORBIDRAFT_02g003840) SORBI_04g029900(SORBIDRAFT_04g029900)
ZMA: 
100191328(GRMZM2G056252) 100273207 100282744 100381639(GRMZM2G161792) 103631986(GRMZM2G174766) 103640919(GRMZM2G169240)
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CSL: 
CVR: 
APRO: 
CME: 
CCP: 
AGO: 
ERC: 
KLA: 
LTH: 
ZRO: 
TDL: 
TDEL_0E01510(TDEL0E01510)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CaO19.118(ODE1) CaO19.12399(ODE2) CaO19.4933(ODE2) CaO19.7765(ODE1)
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
NTE: 
NEUTE1DRAFT116751(NEUTE1DRAFT_116751) NEUTE1DRAFT86398(NEUTE1DRAFT_86398)
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
FPU: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
VDA: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_1200024(AO090010000714) AOR_1_386164(AO090001000224)
ANG: 
ANI_1_742074(An08g05160) ANI_1_962124(An14g06980)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
PBN: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
CNE: 
CNB: 
CGI: 
TMS: 
PPL: 
DSQ: 
PCO: 
SHS: 
HIR: 
PSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MPR: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT113988(AGABI1DRAFT_113988) AGABI1DRAFT122947(AGABI1DRAFT_122947) AGABI1DRAFT131792(AGABI1DRAFT_131792) AGABI1DRAFT60497(AGABI1DRAFT_60497) AGABI1DRAFT75906(AGABI1DRAFT_75906)
ABV: 
AGABI2DRAFT187622(AGABI2DRAFT_187622) AGABI2DRAFT193825(AGABI2DRAFT_193825) AGABI2DRAFT209944(AGABI2DRAFT_209944) AGABI2DRAFT225837(AGABI2DRAFT_225837) AGABI2DRAFT226540(AGABI2DRAFT_226540)
CPUT: 
SLA: 
WSE: 
WIC: 
PFP: 
MGL: 
PGR: 
MLR: 
MBR: 
SRE: 
ACAN: 
TET: 
PTM: 
PTI: 
TPS: 
AAF: 
NGD: 
PIF: 
PSOJ: 
SPAR: 
EHX: 
GTT: 
TBR: 
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
SYW: 
SYD: 
SYX: 
SYNR: 
SYH: 
PMA: 
Pro_1214(desA)
PMM: 
PMT: 
PMN: 
PMI: 
PMB: 
PMC: 
PMF: 
PMG: 
PMH: 
PMJ: 
PME: 
PRC: 
PRM: 
 » show all
Taxonomy
Reference
1  [PMID:2248971]
  Authors
Borgeson CE, de Renobales M, Blomquist GJ.
  Title
Characterization of the delta 12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate.
  Journal
Biochim. Biophys. Acta. 1047 (1990) 135-40.
Reference
2  [PMID:8900454]
  Authors
Lomascolo A, Dubreucq E, Galzy P.
  Title
Study of the delta 12-desaturase system of Lipomyces starkeyi.
  Journal
Lipids. 31 (1996) 253-9.
Reference
3  [PMID:9829122]
  Authors
Tocher DR, Leaver MJ, Hodgson PA.
  Title
Recent advances in the biochemistry and molecular biology of fatty acyl desaturases.
  Journal
Prog. Lipid. Res. 37 (1998) 73-117.
Reference
4  [PMID:15009186]
  Authors
Petrini GA, Altabe SG, Uttaro AD
  Title
Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor.
  Journal
Eur. J. Biochem. 271 (2004) 1079-86.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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