KEGG   ENZYME: 1.14.21.3Help
Entry
EC 1.14.21.3                Enzyme                                 

Name
berbamunine synthase;
(S)-N-methylcoclaurine oxidase (C-O phenol-coupling)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and the other dehydrogenated
BRITE hierarchy
Sysname
(S)-N-methylcoclaurine,NADPH:oxygen oxidoreductase (C-O phenol-coupling)
Reaction(IUBMB)
(S)-N-methylcoclaurine + (R)-N-methylcoclaurine + NADPH + H+ + O2 = berbamunine + NADP+ + 2 H2O [RN:R04694]
Reaction(KEGG)
R04694;
(other) R05210 R05215
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Substrate
(S)-N-methylcoclaurine [CPD:C05176];
(R)-N-methylcoclaurine [CPD:C05243];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
berbamunine [CPD:C05177];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate enzyme (P-450). Forms the bisbenzylisoquinoline alkaloid berbamunine by phenol oxidation of N-methylcoclaurine without the incorporation of oxygen into the product. Reaction of two molecules of (R)-N-methylcoclaurine gives the dimer guattagaumerine.
History
EC 1.14.21.3 created 1999 as EC 1.1.3.34, transferred 2002 to EC 1.14.21.3
Pathway
Isoquinoline alkaloid biosynthesis
Orthology
K13387  
berbamunine synthase
Reference
1  [PMID:8380416]
  Authors
Stadler R, Zenk MH.
  Title
The purification and characterization of a unique cytochrome P-450 enzyme from Berberis stolonifera plant cell cultures.
  Journal
J. Biol. Chem. 268 (1993) 823-31.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
144941-42-4

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