KEGG   ENZYME: 1.14.21.6Help
Entry
EC 1.14.21.6                Enzyme                                 
Name
lathosterol oxidase;
Delta7-sterol Delta5-dehydrogenase;
Delta7-sterol 5-desaturase;
Delta7-sterol-C5(6)-desaturase;
5-DES
Class
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
With NADH or NADPH as one donor, and the other dehydrogenated
BRITE hierarchy
Sysname
5alpha-cholest-7-en-3beta-ol,NAD(P)H:oxygen 5-oxidoreductase
Reaction(IUBMB)
5alpha-cholest-7-en-3beta-ol + NAD(P)H + H+ + O2 = cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O [RN:R03310 R07215]
Reaction(KEGG)
Substrate
5alpha-cholest-7-en-3beta-ol [CPD:C01189];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
cholesta-5,7-dien-3beta-ol [CPD:C01164];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
This enzyme catalyses the introduction of a C5 double bond into the B ring of Delta7-sterols to yield the corresponding Delta5,7-sterols in mammals, yeast and plants [4]. Forms part of the plant sterol biosynthesis pathway.
Pathway
Steroid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K00227  
lathosterol oxidase
Genes
HSA: 
6309(SC5D)
PTR: 
451624(SC5DL)
PPS: 
100979606(SC5DL)
GGO: 
PON: 
100459805(SC5DL)
MCC: 
706827(SC5DL)
MMU: 
235293(Sc5d)
RNO: 
114100(Sc5dl)
CFA: 
610411(SC5DL)
AML: 
FCA: 
101099997(SC5DL)
BTA: 
525154(SC5DL)
SSC: 
100520925
ECB: 
100063543
MDO: 
100030841
SHR: 
100932949(SC5DL)
OAA: 
100073392
GGA: 
100857756
MGP: 
100538497
TGU: 
100226384
ACS: 
100566753
XLA: 
447026(sc5d)
XTR: 
100490845
DRE: 
447891(sc5dl)
TRU: 
101067117
OLA: 
101173647
BFO: 
BRAFLDRAFT_288915
SPU: 
588561
NVE: 
NEMVE_v1g243571
TAD: 
TRIADDRAFT_18639
AQU: 
100635449
ATH: 
AT3G02580(STE1) AT3G02590
ALY: 
ARALYDRAFT_477495 ARALYDRAFT_477496
GMX: 
100782638 100790906
CSV: 
101204738
RCU: 
RCOM_1728170
POP: 
POPTR_670923 POPTR_831136
VVI: 
100257622 100262743 100265283
OSA: 
4325687
DOSA: 
Os01t0134500-01(Os01g0134500)
BDI: 
100823877
SBI: 
SORBI_03g007010(SORBIDRAFT_03g007010)
ZMA: 
100282407 100282855
SMO: 
SELMODRAFT_146325 SELMODRAFT_182646
PPP: 
PHYPADRAFT_135889
CRE: 
CHLREDRAFT_59933
VCN: 
VOLCADRAFT_81225
OLU: 
OSTLU_6297
OTA: 
Ot05g00940
CME: 
CMC055C
SCE: 
YLR056W(ERG3)
AGO: 
AGOS_AFR151C
ERC: 
Ecym_1501
KLA: 
KLLA0F08855g
LTH: 
KLTH0G18304g
PPA: 
PAS_chr1-4_0367
VPO: 
Kpol_1035p33
ZRO: 
ZYRO0F03212g
CGR: 
CAGL0F01793g
NDI: 
NDAI_0C06430(NDAI0C06430)
TPF: 
TPHA_0D00440(TPHA0D00440) TPHA_0P00250(TPHA0P00250)
TBL: 
TBLA_0D02520(TBLA0D02520)
TDL: 
TDEL_0C00670(TDEL0C00670)
KAF: 
KAFR_0D01010(KAFR0D01010)
DHA: 
DEHA2F08866g
PIC: 
PICST_28450(ERG3)
LEL: 
LELG_01797
CAL: 
CaO19.767(ERG3) CaO19.8387(ERG3)
CTP: 
CTRG_04480
CDU: 
CD36_04520(ERG3)
COT: 
CORT_0B06770
YLI: 
YALI0D20878g
CLU: 
CLUG_05120
NCR: 
NCU06207
SMP: 
SMAC_07109 SMAC_08592
PAN: 
PODANSg5469
TTT: 
THITE_64954
MTM: 
MYCTH_112329
MGR: 
MGG_08832
FGR: 
FG02502.1
NHE: 
NECHADRAFT_43685 NECHADRAFT_45966
VAL: 
VDBG_00635 VDBG_08651
SSL: 
SS1G_08964
BFU: 
BC1G_12308
ANI: 
AN6506.2
NFI: 
NFIA_051710 NFIA_094510
AFM: 
AFUA_6G05140
AOR: 
AOR_1_42114(AO090701000022)
ANG: 
ANI_1_28134(An15g00150)
AFV: 
AFLA_054820
ACT: 
ACLA_020750 ACLA_095400
PCS: 
Pc12g04600
CIM: 
CIMG_02610 CIMG_10100
CPW: 
CPC735_037410
PBL: 
PAAG_03651
URE: 
UREG_03145
ABE: 
ARB_04289 ARB_05812
TVE: 
TRV_05362 TRV_06270
AJE: 
HCAG_06888
PNO: 
SNOG_09349
PTE: 
PTT_15444
ZTR: 
MYCGRDRAFT_74506(ERG3)
TML: 
GSTUM_00007875001
SPO: 
SPAC1687.16c(erg31)
CNE: 
CNA05010
CNB: 
CNBA4830
CGI: 
CGB_A5290W
LBC: 
LACBIDRAFT_190564(ERG3)
CCI: 
CC1G_12572
SCM: 
SCHCODRAFT_67790
UMA: 
UM03593.1
MGL: 
MGL_0204
MBR: 
MONBRDRAFT_32318
NGR: 
NAEGRDRAFT_77816
TET: 
TTHERM_00758950 TTHERM_01194720
TBR: 
Tb927.8.3240(Tb08.28L1.250)
TCR: 
473111.10 507853.10
LMA: 
LMJF_23_1300
LIF: 
LINJ_23_1560
LDO: 
LDBPK_231560
LMI: 
LMXM_23_1300
LBZ: 
LBRM_23_1420
PTI: 
PHATRDRAFT_14208
PIF: 
PITG_21426
HCH: 
HCH_01597
 » show all
Taxonomy
Reference
1  [PMID:14189869]
  Authors
DEMPSEY ME, SEATON JD, SCHROEPFER GJ Jr, TROCKMAN RW.
  Title
THE INTERMEDIARY ROLE OF DELTA-5,7-CHOLESTADIEN-3-BETA-OL IN CHOLESTEROL BIOSYNTHESIS.
  Journal
J. Biol. Chem. 239 (1964) 1381-7.
  Organism
Rattus norvegicus [GN:rno]
Reference
2  [PMID:9056262]
  Authors
Nishino H, Nakaya J, Nishi S, Kurosawa T, Ishibashi T.
  Title
Temperature-induced differential kinetic properties between an initial burst and the following steady state in membrane-bound enzymes: studies on lathosterol 5-desaturase.
  Journal
Arch. Biochem. Biophys. 339 (1997) 298-304.
  Organism
Rattus norvegicus [GN:rno]
Reference
3  [PMID:8561508]
  Authors
Taton M, Rahier A.
  Title
Plant sterol biosynthesis: identification and characterization of higher plant delta 7-sterol C5(6)-desaturase.
  Journal
Arch. Biochem. Biophys. 325 (1996) 279-88.
  Organism
Zea mays [GN:zma]
Reference
4  [PMID:10651635]
  Authors
Taton M, Husselstein T, Benveniste P, Rahier A.
  Title
Role of highly conserved residues in the reaction catalyzed by recombinant Delta7-sterol-C5(6)-desaturase studied by site-directed mutagenesis.
  Journal
Biochemistry. 39 (2000) 701-11.
  Organism
Arabidopsis thaliana [GN:ath]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37255-37-1
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