| Entry |
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| Name |
4-methoxybenzoate monooxygenase (O-demethylating);
4-methoxybenzoate 4-monooxygenase (O-demethylating);
4-methoxybenzoate O-demethylase;
p-anisic O-demethylase;
piperonylate-4-O-demethylase
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| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
Miscellaneous
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| Sysname |
4-methoxybenzoate,hydrogen-donor:oxygen oxidoreductase (O-demethylating)
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| Reaction(IUBMB) |
4-methoxybenzoate + AH2 + O2 = 4-hydroxybenzoate + formaldehyde + A + H2O [RN: R01306]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
The bacterial enzyme consists of a ferredoxin-type protein and an iron-sulfur flavoprotein (FMN). Also acts on 4-ethoxybenzoate, N-methyl-4-aminobenzoate and toluate. The fungal enzyme acts best on veratrate.
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| Pathway |
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| Reference |
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| Authors |
Bernhardt FH, Nastainczyk W, Seydewitz V. |
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| Title |
Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida. |
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| Journal |
Eur. J. Biochem. 72 (1977) 107-15. |
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| Organism |
Pseudomonas putida |
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| Reference |
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| Authors |
Paszczynski A, Trojanowski J. |
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| Title |
An affinity-column procedure for the purification of veratrate O-demethylase from fungi. |
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| Journal |
Microbios. 18 (1977) 111-21. |
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| Organism |
Chaetomium piluliferum, Xerocomus badius |
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| Reference |
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| Authors |
Twilfer H, Bernhardt FH, Gersonde K. |
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| Title |
An electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from Pseudomonas putida. |
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| Journal |
Eur. J. Biochem. 119 (1981) 595-602. |
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| Organism |
Pseudomonas putida |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: UM-BBD (Biocatalysis/Biodegradation Database): BRENDA, the Enzyme Database: CAS: 37256-78-3 |
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