KEGG   ENZYME: 1.14.99.15Help
Entry
EC 1.14.99.15               Enzyme                                 

Name
4-methoxybenzoate monooxygenase (O-demethylating);
4-methoxybenzoate 4-monooxygenase (O-demethylating);
4-methoxybenzoate O-demethylase;
p-anisic O-demethylase;
piperonylate-4-O-demethylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
BRITE hierarchy
Sysname
4-methoxybenzoate,hydrogen-donor:oxygen oxidoreductase (O-demethylating)
Reaction(IUBMB)
4-methoxybenzoate + AH2 + O2 = 4-hydroxybenzoate + formaldehyde + A + H2O [RN:R01306]
Reaction(KEGG)
Substrate
4-methoxybenzoate [CPD:C02519];
AH2 [CPD:C00030];
O2 [CPD:C00007]
Product
4-hydroxybenzoate [CPD:C00156];
formaldehyde [CPD:C00067];
A [CPD:C00028];
H2O [CPD:C00001]
Comment
The bacterial enzyme consists of a ferredoxin-type protein and an iron-sulfur flavoprotein (FMN). Also acts on 4-ethoxybenzoate, N-methyl-4-aminobenzoate and toluate. The fungal enzyme acts best on veratrate.
History
EC 1.14.99.15 created 1972
Pathway
Benzoate degradation
Reference
1  [PMID:188654]
  Authors
Bernhardt FH, Nastainczyk W, Seydewitz V.
  Title
Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida.
  Journal
Eur. J. Biochem. 72 (1977) 107-15.
  Organism
Pseudomonas putida
Reference
2  [PMID:25369]
  Authors
Paszczynski A, Trojanowski J.
  Title
An affinity-column procedure for the purification of veratrate O-demethylase from fungi.
  Journal
Microbios. 18 (1977) 111-21.
  Organism
Chaetomium piluliferum, Xerocomus badius
Reference
3  [PMID:6273164]
  Authors
Twilfer H, Bernhardt FH, Gersonde K.
  Title
An electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from Pseudomonas putida.
  Journal
Eur. J. Biochem. 119 (1981) 595-602.
  Organism
Pseudomonas putida
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
37256-78-3

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