KEGG   ENZYME: 1.14.99.22Help
Entry
EC 1.14.99.22               Enzyme                                 

Name
ecdysone 20-monooxygenase;
alpha-ecdysone C-20 hydroxylase;
ecdysone 20-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
BRITE hierarchy
Sysname
Ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating)
Reaction(IUBMB)
ecdysone + AH2 + O2 = 20-hydroxyecdysone + A + H2O [RN:R02374]
Reaction(KEGG)
Substrate
ecdysone [CPD:C00477];
AH2 [CPD:C00030];
O2 [CPD:C00007]
Product
20-hydroxyecdysone [CPD:C02633];
A [CPD:C00028];
H2O [CPD:C00001]
Comment
An enzyme from insect fat body or malpighian tubules involving a heme-thiolate protein (P-450). NADPH can act as ultimate hydrogen donor.
History
EC 1.14.99.22 created 1978
Pathway
Insect hormone biosynthesis
Biosynthesis of secondary metabolites
Orthology
K10723  
ecdysone 20-monooxygenase
Genes
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
411057(Cyp314a1)
NVI: 
100115247(CYP314A1)
TCA: 
661451(Cyp314a1)
BMOR: 
100127102(Cyp314a1)
API: 
PHU: 
ISC: 
 » show all
Taxonomy
Reference
1  [PMID:606249]
  Authors
Johnson P, Rees HH.
  Title
The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria.
  Journal
Biochem. J. 168 (1977) 513-20.
  Organism
Schistocerca gregaria
Reference
2  [PMID:5286]
  Authors
Nigg HN, Svoboda JA, Thompson MJ, Dutky SR, Kaplanis JN, Robbins WE.
  Title
Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.).
  Journal
Experientia. 32 (1976) 438-9.
  Organism
Manduca sexta
Reference
3  [PMID:488526]
  Authors
Smith SL, Bollenbacher WE, Cooper DY, Schleyer H, Wielgus JJ, Gilbert LI.
  Title
Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase.
  Journal
Mol. Cell. Endocrinol. 15 (1979) 111-33.
  Organism
Manduca sexta
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
55071-97-1

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