KEGG   ENZYME: 1.14.99.42Help
Entry
EC 1.14.99.42               Enzyme                                 

Name
zeaxanthin 7,8-dioxygenase;
zeaxanthin 7,8(7',8')-cleavage dioxygenase;
CsZCD (incorrect);
CCD2 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
BRITE hierarchy
Sysname
zeaxanthin:oxygen oxidoreductase (7,8-cleaving)
Reaction(IUBMB)
zeaxanthin + 2 O2 = crocetin dialdehyde + 2 (3S)-3-hydroxycyclocitral (overall reaction) [RN:R09603];
(1a) zeaxanthin + O2 = beta-citraurin + (3S)-3-hydroxycyclocitral [RN:R10843];
(1b) beta-citraurin + O2 = crocetin dialdehyde + (3S)-3-hydroxycyclocitral [RN:R10844]
Reaction(KEGG)
Substrate
zeaxanthin [CPD:C06098];
O2 [CPD:C00007];
beta-citraurin [CPD:C20894]
Product
crocetin dialdehyde [CPD:C19730];
(3S)-3-hydroxycyclocitral [CPD:C19731];
beta-citraurin [CPD:C20894]
Comment
The enzyme, characterized from the plant Crocus sativus, acts twice on zeaxanthin, cleaving 3-hydroxycyclocitral off each 3-hydroxy end group. It is part of the zeaxanthin degradation pathway in that organism, leading to the different compounds that impart the color, flavor and aroma of the saffron spice.
History
EC 1.14.99.42 created 2011, modified 2014
Reference
1  [PMID:25097262]
  Authors
Frusciante S, Diretto G, Bruno M, Ferrante P, Pietrella M, Prado-Cabrero A, Rubio-Moraga A, Beyer P, Gomez-Gomez L, Al-Babili S, Giuliano G
  Title
Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 111 (2014) 12246-51.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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