KEGG   ENZYME: 1.14.99.44Help
Entry
EC 1.14.99.44               Enzyme                                 
Name
diapolycopene oxygenase;
crtP (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
Miscellaneous
BRITE hierarchy
Sysname
4,4'-diapolycopene,AH2:oxygen oxidoreductase (4,4'-hydroxylating)
Reaction(IUBMB)
4,4'-diapolycopene + 4 AH2 + 4 O2 = 4,4'-diapolycopenedial + 4 A + 6 H2O [RN:R09671]
Reaction(KEGG)
Substrate
4,4'-diapolycopene [CPD:C19797];
AH2 [CPD:C00030];
O2 [CPD:C00007]
Product
4,4'-diapolycopenedial [CPD:C19798];
A [CPD:C00028];
H2O [CPD:C00001]
Comment
Little activity with neurosporene or lycopene. Involved in the biosynthesis of C30 carotenoids such as staphyloxanthin. The enzyme oxidizes each
methyl group to the hydroxymethyl and then a dihydroxymethyl group,
followed by the spontaneous loss of water to give an aldehyde group.
Pathway
Carotenoid biosynthesis
Orthology
K10210  
diapolycopene oxygenase
Genes
BMH: 
SAU: 
SAV: 
SAW: 
SAH: 
SAJ: 
SAM: 
SAS: 
SAR: 
SAC: 
SAX: 
USA300HOU_2559
SAA: 
SAUSA300_2501
SAO: 
SAOUHSC_02881
SAE: 
NWMN_2464(crtI)
SAD: 
SAAV_2630
SUU: 
M013TW_2538
SUV: 
SAVC_11700
SUE: 
SAOV_2609c
SUJ: 
SAA6159_02460(crtI)
SUK: 
SAA6008_02603(crtI)
SUC: 
ECTR2_2417(crtI)
SUT: 
SAT0131_02775
SUQ: 
HMPREF0772_10625(crtI)
SUZ: 
MS7_2571(crtP)
SUD: 
ST398NM01_2615
SUX: 
SAEMRSA15_24670
SUW: 
SATW20_26860
SUG: 
SAPIG2615
SUF: 
SARLGA251_23390
SAB: 
SAB2437c
SUY: 
SA2981_2501
SAUB: 
C248_2622
SAUM: 
BN843_26010
SHA: 
SH0488
SLG: 
SLGD_00185
SLN: 
SLUG_01830
HHD: 
HBHAL_1313
SIV: 
SSIL_3759
ECAS: 
ECBG_01335
 » show all
Taxonomy
Reference
1  [PMID:15850982]
  Authors
Mijts BN, Lee PC, Schmidt-Dannert C
  Title
Identification of a carotenoid oxygenase synthesizing acyclic xanthophylls: combinatorial biosynthesis and directed evolution.
  Journal
Chem. Biol. 12 (2005) 453-60.
  Organism
Staphylococcus aureus
Reference
2  [PMID:15933032]
  Authors
Tao L, Schenzle A, Odom JM, Cheng Q
  Title
Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene dialdehyde.
  Journal
Appl. Environ. Microbiol. 71 (2005) 3294-301.
  Organism
Staphylococcus aureus
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system