| Entry |
|
|---|
| Name |
leucoanthocyanidin reductase;
leucocyanidin reductase |
|---|
| Class |
Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor
 |
|---|
| Sysname |
(2R,3S)-catechin:NADP+ 4-oxidoreductase |
|---|
| Reaction(IUBMB) |
(2R,3S)-catechin + NADP+ + H2O = 2,3-trans-3,4-cis-leucocyanidin +
NADPH + H+ [RN:R06532] |
|---|
| Reaction(KEGG) |
R06532;
(other) R06533 R06615
 |
|---|
| Substrate |
(2R,3S)-catechin [CPD:C06562];
NADP+ [CPD:C00006];
H2O [CPD:C00001] |
|---|
| Product |
2,3-trans-3,4-cis-leucocyanidin [CPD:C05906];
NADPH [CPD:C00005];
H+ [CPD:C00080] |
|---|
| Comment |
The enzyme catalyses the synthesis of catechin, catechin-4beta-ol
(leucocyanidin) and the related flavan-3-ols afzelechin and
gallocatechin, which are initiating monomers in the synthesis of
plant polymeric proanthocyanidins or condensed tannins. While
2,3-trans-3,4-cis-leucocyanidin is the preferred flavan-3,4-diol
substrate, 2,3-trans-3,4-cis-leucodelphinidin and
2,3-trans-3,4-cis-leucopelargonidin can also act as substrates, but
more slowly. NADH can replace NADPH but is oxidized more slowly. |
|---|
| Pathway |
ec00941 Flavonoid biosynthesis
ec01110 Biosynthesis of secondary metabolites |
|---|
| Orthology |
K13081 leucoanthocyanidin reductase |
|---|
| Genes |
POP: POPTR_566672(LAR2) POPTR_835080(LAR3)
VVI: 100232982(LAR1) 100233112(LAR2)
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:8470799]
Tanner GJ, Kristiansen KN.
Synthesis of 3,4-cis-[3H]leucocyanidin and enzymatic reduction to
catechin.
Anal. Biochem. 209 (1993) 274-7.
Onobrychis viciifolia |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:12788945]
Tanner GJ, Francki KT, Abrahams S, Watson JM, Larkin PJ, Ashton AR.
Proanthocyanidin biosynthesis in plants. Purification of legume
leucoanthocyanidin reductase and molecular cloning of its cDNA.
J. Biol. Chem. 278 (2003) 31647-56.
Desmodium uncinatum |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.17.1.3
IUBMB Enzyme Nomenclature: 1.17.1.3
ExPASy - ENZYME nomenclature database: 1.17.1.3
BRENDA, the Enzyme Database: 1.17.1.3
CAS: 776323-46-7 |
|---|
