KEGG   ENZYME: 1.17.1.5Help
Entry
EC 1.17.1.5                 Enzyme                                 

Name
nicotinate dehydrogenase;
nicotinic acid hydroxylase;
nicotinate hydroxylase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
nicotinate:NADP+ 6-oxidoreductase (hydroxylating)
Reaction(IUBMB)
nicotinate + H2O + NADP+ = 6-hydroxynicotinate + NADPH + H+ [RN:R01720]
Reaction(KEGG)
Substrate
nicotinate [CPD:C00253];
H2O [CPD:C00001];
NADP+ [CPD:C00006]
Product
6-hydroxynicotinate [CPD:C01020];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
A flavoprotein containing non-heme iron. The enzyme is capable of acting on a variety of nicotinate analogues to varying degrees, including pyrazine-2-carboxylate, pyrazine 2,3-dicarboxylate, trigonelline and 6-methylnicotinate. The enzyme from Clostridium barkeri also possesses a catalytically essential, labile selenium that can be removed by reaction with cyanide.
History
EC 1.17.1.5 created 1972 as EC 1.5.1.13, transferred 2004 to EC 1.17.1.5
Pathway
Nicotinate and nicotinamide metabolism
Microbial metabolism in diverse environments
Orthology
K20445  
nicotinate dehydrogenase FAD-subunit
K20446  
nicotinate dehydrogenase small FeS subunit
K20447  
nicotinate dehydrogenase large molybdopterin subunit
K20448  
nicotinate dehydrogenase medium molybdopterin subunit
Genes
DTI: 
GGH: 
GHH_c21850(pucD2)
GEA: 
CPAT: 
CLPA_c02860(ndhF1) CLPA_c02870(ndhS1) CLPA_c02880(ndhL)
CPAE: 
AMT: 
AOE: 
DOR: 
DAI: 
DMI: 
CHY: 
CHY_0690(coxM)
TEP: 
TAE: 
MTA: 
NTH: 
HHL: 
IPO: 
PMO: 
 » show all
Taxonomy
Reference
1  [PMID:4388026]
  Authors
Holcenberg JS, Stadtman ER.
  Title
Nicotinic acid metabolism. 3. Purification and properties of a nicotinic acid hydroxylase.
  Journal
J. Biol. Chem. 244 (1969) 1194-203.
Reference
2  [PMID:8555176]
  Authors
Gladyshev VN, Khangulov SV, Stadtman TC.
  Title
Properties of the selenium- and molybdenum-containing nicotinic acid hydroxylase from Clostridium barkeri.
  Journal
Biochemistry. 35 (1996) 212-23.
  Sequence
Reference
3  [PMID:8278371]
  Authors
Gladyshev VN, Khangulov SV, Stadtman TC.
  Title
Nicotinic acid hydroxylase from Clostridium barkeri: electron paramagnetic resonance studies show that selenium is coordinated with molybdenum in the catalytically active selenium-dependent enzyme.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 232-6.
Reference
4  [PMID:6838209]
  Authors
Dilworth GL.
  Title
Occurrence of molybdenum in the nicotinic acid hydroxylase from Clostridium barkeri.
  Journal
Arch. Biochem. Biophys. 221 (1983) 565-9.
Reference
5  [PMID:7181513]
  Authors
Dilworth GL.
  Title
Properties of the selenium-containing moiety of nicotinic acid hydroxylase from Clostridium barkeri.
  Journal
Arch. Biochem. Biophys. 219 (1982) 30-8.
Reference
6
  Authors
Nagel, M. and Andreesen, J.R.
  Title
Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini.
  Journal
Arch. Microbiol. 154 (1990) 605-613.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9059-03-4

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