| Entry |
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| Name |
nicotinate dehydrogenase;
nicotinic acid hydroxylase;
nicotinate hydroxylase
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| Class |
Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor
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| Sysname |
nicotinate:NADP+ 6-oxidoreductase (hydroxylating)
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| Reaction(IUBMB) |
nicotinate + H2O + NADP+ = 6-hydroxynicotinate + NADPH + H+ [RN: R01720]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
A flavoprotein containing non-heme iron. The enzyme is capable of acting on a variety of nicotinate analogues to varying degrees, including pyrazine-2-carboxylate, pyrazine 2,3-dicarboxylate, trigonelline and 6-methylnicotinate. The enzyme from Clostridium barkeri also possesses a catalytically essential, labile selenium that can be removed by reaction with cyanide.
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| Pathway |
| Nicotinate and nicotinamide metabolism |
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| Reference |
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| Authors |
Holcenberg JS, Stadtman ER. |
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| Title |
Nicotinic acid metabolism. 3. Purification and properties of a nicotinic acid hydroxylase. |
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| Journal |
J. Biol. Chem. 244 (1969) 1194-203. |
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| Organism |
Clostridium sp. |
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| Reference |
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| Authors |
Gladyshev VN, Khangulov SV, Stadtman TC. |
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| Title |
Properties of the selenium- and molybdenum-containing nicotinic acid hydroxylase from Clostridium barkeri. |
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| Journal |
Biochemistry. 35 (1996) 212-23. |
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| Organism |
Clostridium barkeri |
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| Reference |
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| Authors |
Gladyshev VN, Khangulov SV, Stadtman TC. |
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| Title |
Nicotinic acid hydroxylase from Clostridium barkeri: electron paramagnetic resonance studies show that selenium is coordinated with molybdenum in the catalytically active selenium-dependent enzyme. |
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| Journal |
Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 232-6. |
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| Organism |
Clostridium barkeri |
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| Reference |
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| Authors |
Dilworth GL. |
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| Title |
Occurrence of molybdenum in the nicotinic acid hydroxylase from Clostridium barkeri. |
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| Journal |
Arch. Biochem. Biophys. 221 (1983) 565-9. |
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| Organism |
Clostridium barkeri |
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| Reference |
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| Authors |
Dilworth GL. |
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| Title |
Properties of the selenium-containing moiety of nicotinic acid hydroxylase from Clostridium barkeri. |
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| Journal |
Arch. Biochem. Biophys. 219 (1982) 30-8. |
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| Organism |
Clostridium barkeri |
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| Reference |
6 |
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| Authors |
Nagel, M. and Andreesen, J.R. |
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| Title |
Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini. |
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| Journal |
Arch. Microbiol. 154 (1990) 605-613. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 9059-03-4 |
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