| Entry |
|
| Name |
4-cresol dehydrogenase (hydroxylating);
p-cresol-(acceptor) oxidoreductase (hydroxylating);
p-cresol methylhydroxylase |
| Class |
Oxidoreductases;
Acting on CH or CH2 groups;
With other acceptors
 |
| Sysname |
4-cresol:acceptor oxidoreductase (methyl-hydroxylating) |
| Reaction(IUBMB) |
4-cresol + acceptor + H2O = 4-hydroxybenzaldehyde + reduced acceptor
[RN:R07223] |
| Reaction(KEGG) |
R07223 > R02675
 |
| Substrate |
4-cresol [CPD:C01468];
acceptor [CPD:C00028];
H2O [CPD:C00001] |
| Product |
4-hydroxybenzaldehyde [CPD:C00633];
reduced acceptor [CPD:C00030] |
| Cofactor |
FAD [CPD:C00016];
Cytochrome c [CPD:C00524] |
| Comment |
A flavocytochrome c (FAD). Phenazine methosulfate can act as
acceptor. A quinone methide is probably formed as intermediate. The
first hydroxylation forms 4-hydroxybenzyl alcohol; a second
hydroxylation converts this into 4-hydroxybenzaldehyde. |
| Pathway |
PATH: ec00622 Toluene and xylene degradation
PATH: ec01100 Metabolic pathways |
| Orthology |
KO: K05797 4-cresol dehydrogenase (hydroxylating) |
| Genes |
PAT: Patl_1202
NOC: Noc_2406
NET: Neut_1656
EBA: ebA3165(pchF)
GME: Gmet_2125
RRU: Rru_A3112
 |
| Structures |
PDB: 1DII 1DIQ 1WVE 1WVF |
Reference Authors Title
Journal Organism
|
1 [PMID:588247]
Hopper DJ, Taylor DG.
The purification and properties of p-cresol-(acceptor)
oxidoreductase (hydroxylating), a flavocytochrome from Pseudomonas
putida.
Biochem. J. 167 (1977) 155-62.
Pseudomonas putida |
Reference Authors Title
Journal Organism
|
2 [PMID:7391034]
McIntire W, Edmondson DE, Singer TP, Hopper DJ.
8 alpha-O-Tyrosyl-FAD: a new form of covalently bound flavin from
p-cresol methylhydroxylase.
J. Biol. Chem. 255 (1980) 6553-5.
Pseudomonas putida |
| Other DBs |
ExplorEnz - The Enzyme Database: 1.17.99.1
IUBMB Enzyme Nomenclature: 1.17.99.1
ExPASy - ENZYME nomenclature database: 1.17.99.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.17.99.1
BRENDA, the Enzyme Database: 1.17.99.1
CAS: 66772-07-4 |