KEGG   ENZYME: 1.17.99.1Help
Entry
EC 1.17.99.1                Enzyme                                 
Name
4-methylphenol dehydrogenase (hydroxylating);
p-cresol-(acceptor) oxidoreductase (hydroxylating);
p-cresol methylhydroxylase;
4-cresol dehydrogenase (hydroxylating)
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With other acceptors
BRITE hierarchy
Sysname
4-methylphenol:acceptor oxidoreductase (methyl-hydroxylating)
Reaction(IUBMB)
4-methylphenol + acceptor + H2O = 4-hydroxybenzaldehyde + reduced acceptor [RN:R07223]
Reaction(KEGG)
Substrate
4-methylphenol [CPD:C01468];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Product
4-hydroxybenzaldehyde [CPD:C00633];
reduced acceptor [CPD:C00030]
Comment
A flavocytochrome c (FAD). Phenazine methosulfate can act as acceptor. A quinone methide is probably formed as intermediate. The first hydroxylation forms 4-hydroxybenzyl alcohol; a second hydroxylation converts this into 4-hydroxybenzaldehyde.
Pathway
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K05797  
4-cresol dehydrogenase (hydroxylating)
Genes
PAT: 
NOC: 
NHL: 
OCE: 
BXE: 
BPH: 
BPY: 
BUG: 
BGE: 
BGF: 
BYI: 
BYI23_B003260(pchF)
AXY: 
AXYL_04709
PNA: 
Pnap_3838
CTT: 
CtCNB1_1149
NEU: 
NE0741
NET: 
Neut_1656
NIT: 
NAL212_2695
NMU: 
Nmul_A1475
EBA: 
ebA3165(pchF) ebA5380
TMZ: 
Tmz1t_1840
SKU: 
Sulku_2563
GME: 
Gmet_2125(pcmI)
GEO: 
Geob_0119
GEM: 
GM21_2847
SUR: 
STAUR_5202
NAR: 
Saro_3479 Saro_3875
NPP: 
PP1Y_AT15495
SSY: 
SLG_38080
RRU: 
Rru_A3112
RHA: 
RHA1_ro03282
MMAR: 
MODMU_2955(vaoA)
NDE: 
NIDE0022(pchF)
 » show all
Taxonomy
Reference
1  [PMID:588247]
  Authors
Hopper DJ, Taylor DG.
  Title
The purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from Pseudomonas putida.
  Journal
Biochem. J. 167 (1977) 155-62.
  Organism
Pseudomonas putida
Reference
2  [PMID:7391034]
  Authors
McIntire W, Edmondson DE, Singer TP, Hopper DJ.
  Title
8 alpha-O-Tyrosyl-FAD: a new form of covalently bound flavin from p-cresol methylhydroxylase.
  Journal
J. Biol. Chem. 255 (1980) 6553-5.
  Organism
Pseudomonas putida
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
66772-07-4
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